Matches in SemOpenAlex for { <https://semopenalex.org/work/W1973668045> ?p ?o ?g. }
- W1973668045 endingPage "344" @default.
- W1973668045 startingPage "333" @default.
- W1973668045 abstract "The nucleotide sequence of a 7-kbp EcoRI fragment of PHT plasmid in Pseudomonas putida encoding the enzymes in the initial step of phthalate degradation (the Pht+ phenotype) revealed five open reading frames (ORFs), pht1 to pht5, with the same orientation. The protein deduced from pht1 showed 17.4% similarity in amino acid sequence with the glycerol-3-phosphate transporter of Escherichia coli, that of pht2 showed 39.2% similarity with VanB protein (vanillate monooxygenase reductase) and that of pht3 showed 20.8% similarity with VanA (vanillate monooxygenase) of Pseudomonas sp. The proteins encoded by pht4 and pht5 showed no appreciable similarity with any proteins in a database. Eight Pht− mutants with a Tn5 insertion in pht1, and one with a similar insertion in the pht5 ORF were isolated. These pht1− and pht5− mutants, and two other Pht− mutants induced by UV irradiation of a Pht+ strain of Pseudomonas testosteroni, were examined by complementation tests with several segments of the 7-kbp pht fragment and feeding experiments with phthalate, 4,5-dihydro-4,5-dihydroxyphthalate, 4,5-dihydroxyphthalate, and protocatechuate. The results of these experiments and similarity searches suggested that pht2 encodes phthalate oxygenase reductase, pht3 encodes phthalate oxygenase, pht4 encodes 4,5-dihydro-4,5-dihydroxyphthalate dehydrogenase, and pht5 encodes 4,5-dihydroxyphthalate decarboxylase. The Pht1 protein was suggested to function as a positive regulator for expression of the other pht genes or as a phthalate transporter. Three A : T rich regions, which may have promoter function, were found in the upstream regions of pht1, pht3, and pht5. Northern blot analyses suggested that transcriptions of pht1 and pht2 are low level and under relaxed control of phthalate. The transcriptions of pht3, pht4, and pht5 might be induced by phthalate." @default.
- W1973668045 created "2016-06-24" @default.
- W1973668045 creator A5006945451 @default.
- W1973668045 creator A5047524684 @default.
- W1973668045 creator A5067066753 @default.
- W1973668045 creator A5068999232 @default.
- W1973668045 creator A5082482679 @default.
- W1973668045 date "1992-01-01" @default.
- W1973668045 modified "2023-10-17" @default.
- W1973668045 title "Genes in PHT plasmid encoding the initial degradation pathway of phthalate in Pseudomonas putida" @default.
- W1973668045 cites W1221386567 @default.
- W1973668045 cites W1480704794 @default.
- W1973668045 cites W1551462053 @default.
- W1973668045 cites W1555021163 @default.
- W1973668045 cites W1562974039 @default.
- W1973668045 cites W1566515097 @default.
- W1973668045 cites W1582454623 @default.
- W1973668045 cites W1911203598 @default.
- W1973668045 cites W1955669359 @default.
- W1973668045 cites W1990490749 @default.
- W1973668045 cites W1993895344 @default.
- W1973668045 cites W1997602579 @default.
- W1973668045 cites W2016770227 @default.
- W1973668045 cites W2028954926 @default.
- W1973668045 cites W2032462421 @default.
- W1973668045 cites W2034248623 @default.
- W1973668045 cites W2034681771 @default.
- W1973668045 cites W2050499271 @default.
- W1973668045 cites W2062030976 @default.
- W1973668045 cites W2063231457 @default.
- W1973668045 cites W2067124267 @default.
- W1973668045 cites W2115699231 @default.
- W1973668045 cites W2116414322 @default.
- W1973668045 cites W2123870929 @default.
- W1973668045 cites W2140158396 @default.
- W1973668045 cites W2168713954 @default.
- W1973668045 cites W53416822 @default.
- W1973668045 cites W65938566 @default.
- W1973668045 cites W9554184 @default.
- W1973668045 doi "https://doi.org/10.1016/0922-338x(92)90028-s" @default.
- W1973668045 hasPublicationYear "1992" @default.
- W1973668045 type Work @default.
- W1973668045 sameAs 1973668045 @default.
- W1973668045 citedByCount "117" @default.
- W1973668045 countsByYear W19736680452012 @default.
- W1973668045 countsByYear W19736680452013 @default.
- W1973668045 countsByYear W19736680452014 @default.
- W1973668045 countsByYear W19736680452015 @default.
- W1973668045 countsByYear W19736680452016 @default.
- W1973668045 countsByYear W19736680452017 @default.
- W1973668045 countsByYear W19736680452018 @default.
- W1973668045 countsByYear W19736680452019 @default.
- W1973668045 countsByYear W19736680452020 @default.
- W1973668045 countsByYear W19736680452021 @default.
- W1973668045 countsByYear W19736680452022 @default.
- W1973668045 countsByYear W19736680452023 @default.
- W1973668045 crossrefType "journal-article" @default.
- W1973668045 hasAuthorship W1973668045A5006945451 @default.
- W1973668045 hasAuthorship W1973668045A5047524684 @default.
- W1973668045 hasAuthorship W1973668045A5067066753 @default.
- W1973668045 hasAuthorship W1973668045A5068999232 @default.
- W1973668045 hasAuthorship W1973668045A5082482679 @default.
- W1973668045 hasConcept C104317684 @default.
- W1973668045 hasConcept C129075420 @default.
- W1973668045 hasConcept C143065580 @default.
- W1973668045 hasConcept C149011108 @default.
- W1973668045 hasConcept C153911025 @default.
- W1973668045 hasConcept C167625842 @default.
- W1973668045 hasConcept C188082640 @default.
- W1973668045 hasConcept C203075996 @default.
- W1973668045 hasConcept C22744801 @default.
- W1973668045 hasConcept C2780543182 @default.
- W1973668045 hasConcept C44312359 @default.
- W1973668045 hasConcept C47289529 @default.
- W1973668045 hasConcept C547475151 @default.
- W1973668045 hasConcept C55493867 @default.
- W1973668045 hasConcept C84148353 @default.
- W1973668045 hasConcept C86803240 @default.
- W1973668045 hasConceptScore W1973668045C104317684 @default.
- W1973668045 hasConceptScore W1973668045C129075420 @default.
- W1973668045 hasConceptScore W1973668045C143065580 @default.
- W1973668045 hasConceptScore W1973668045C149011108 @default.
- W1973668045 hasConceptScore W1973668045C153911025 @default.
- W1973668045 hasConceptScore W1973668045C167625842 @default.
- W1973668045 hasConceptScore W1973668045C188082640 @default.
- W1973668045 hasConceptScore W1973668045C203075996 @default.
- W1973668045 hasConceptScore W1973668045C22744801 @default.
- W1973668045 hasConceptScore W1973668045C2780543182 @default.
- W1973668045 hasConceptScore W1973668045C44312359 @default.
- W1973668045 hasConceptScore W1973668045C47289529 @default.
- W1973668045 hasConceptScore W1973668045C547475151 @default.
- W1973668045 hasConceptScore W1973668045C55493867 @default.
- W1973668045 hasConceptScore W1973668045C84148353 @default.
- W1973668045 hasConceptScore W1973668045C86803240 @default.
- W1973668045 hasIssue "6" @default.
- W1973668045 hasLocation W19736680451 @default.
- W1973668045 hasOpenAccess W1973668045 @default.
- W1973668045 hasPrimaryLocation W19736680451 @default.