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- W1994368045 abstract "Collagen types synthesized by murine bone marrow cells were studied and the effect of lithium chloride on collagen biosynthesis in vitro was investigated. In the liquid culture system used, an adherent, mixed cell population supports hemopoiesis. Radioactive labeling of cell cultures and subsequent fractionation with ammonium sulfate, enzyme digestion, immune precipitation, and gel electrophoresis indicated that the bone marrow cells synthesized precursors to collagen types I, III, and IV, and fibronectin. A previously undescribed molecule or fragment with an apparent molecular weight of 17,000 daltons that was susceptible to bacterial collagenase and containing no interchain disulfide bonds was also identified in the culture media of both control and lithium-treated cells. Lithium treatment did not affect the types of collagen synthesized, although the relative proportions of collagen types may differ from controls. However, lithium does have an effect on the appearance of some, as yet unidentified, non-collagenous components in the cell culture media." @default.
- W1994368045 created "2016-06-24" @default.
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- W1994368045 date "1986-06-01" @default.
- W1994368045 modified "2023-09-23" @default.
- W1994368045 title "Collagen synthesis by murine bone marrow cell culture" @default.
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- W1994368045 doi "https://doi.org/10.1002/jcp.1041270307" @default.
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