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• W1994915511 abstract "To determine the direct effects of cigarette smoke (CS) on human endometrial surface epithelial cell line proliferation.In vitro study using HES cells and primary human endometrial cells.University-based academic center.Premenopausal women in the proliferative phase of the cycle.The HES cells and primary human endometrial cells were exposed to cigarette smoke-saturated solution.Cell proliferation and expression of different isoforms of nitric oxide synthase.Cigarette smoke inhibited HES cell proliferation in a dose- and time-dependant manner. The inhibitory effect of CS was blocked by hemoglobin and enhanced by L-arginine (L-Arg). Cigarette smoking and nicotine stimulated the expression of endothelial NO synthase (eNOS) and inducible NO synthase (iNOS) whereas benzo[a]pyrene (BP) only stimulated the expression of eNOS in HES cells. Cigarette smoke stimulated the expression of eNOS/iNOS in primary human endometrial cells, comprised of epithelial and stromal cells. The effect of CS on eNOS/iNOS expression in HES cells was blocked by ascorbic acid but not by glutathione.Cigarette smoke inhibits endometrial cell proliferation through a nitric oxide-mediated pathway." @default.
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• W1994915511 date "2010-01-01" @default.
• W1994915511 modified "2023-09-28" @default.
• W1994915511 title "Cigarette smoke inhibits endometrial epithelial cell proliferation through a nitric oxide–mediated pathway" @default.
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• W1994915511 doi "https://doi.org/10.1016/j.fertnstert.2008.09.074" @default.
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