FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1998045383> ?p ?o ?g. }

• W1998045383 endingPage "1533" @default.
• W1998045383 startingPage "1528" @default.
• W1998045383 abstract "To obtain quantitative information on the size and dynamics of unfolded proteins we combined single-molecule lifetime and intensity FRET measurements with molecular simulations. We compared the unfolded states of the 64-residue, α/β protein L and the 66-residue, all-β cold-shock protein Csp Tm . The average radius of gyration ( R g ) calculated from FRET data on freely diffusing molecules was identical for the two unfolded proteins at guanidinium chloride concentrations >3 M, and the FRET-derived R g of protein L agreed well with the R g previously measured by equilibrium small-angle x-ray scattering. As the denaturant concentration was lowered, the mean FRET efficiency of the unfolded subpopulation increased, signaling collapse of the polypeptide chain, with protein L being slightly more compact than Csp Tm . A decrease in R g with decreasing denaturant was also observed in all-atom molecular dynamics calculations in explicit water/urea solvent, and Langevin simulations of a simplified representation of the polypeptide suggest that collapse can result from either increased interresidue attraction or decreased excluded volume. In contrast to both the FRET and simulation results, previous time-resolved small-angle x-ray scattering experiments showed no collapse for protein L. Analysis of the donor fluorescence decay of the unfolded subpopulation of both proteins gives information about the end-to-end chain distribution and suggests that chain dynamics is slow compared with the donor life-time of ≈2 ns, whereas the bin-size independence of the small excess width above the shot noise for the FRET efficiency distributions may result from incomplete conformational averaging on even the 1-ms time scale." @default.
• W1998045383 created "2016-06-24" @default.
• W1998045383 creator A5032949743 @default.
• W1998045383 creator A5059620610 @default.
• W1998045383 creator A5066209297 @default.
• W1998045383 creator A5074112300 @default.
• W1998045383 creator A5075318212 @default.
• W1998045383 date "2007-01-30" @default.
• W1998045383 modified "2023-09-23" @default.
• W1998045383 title "Characterizing the unfolded states of proteins using single-molecule FRET spectroscopy and molecular simulations" @default.
• W1998045383 cites W1499741065 @default.
• W1998045383 cites W1580614613 @default.
• W1998045383 cites W1593425281 @default.
• W1998045383 cites W1600015000 @default.
• W1998045383 cites W1972327318 @default.
• W1998045383 cites W1973706355 @default.
• W1998045383 cites W1978129474 @default.
• W1998045383 cites W1980014445 @default.
• W1998045383 cites W1984758140 @default.
• W1998045383 cites W1991252112 @default.
• W1998045383 cites W1998030898 @default.
• W1998045383 cites W2003204624 @default.
• W1998045383 cites W2007318191 @default.
• W1998045383 cites W2015811557 @default.
• W1998045383 cites W2030745215 @default.
• W1998045383 cites W2032311486 @default.
• W1998045383 cites W2041505869 @default.
• W1998045383 cites W2045439258 @default.
• W1998045383 cites W2050463871 @default.
• W1998045383 cites W2063107779 @default.
• W1998045383 cites W2064589158 @default.
• W1998045383 cites W2064901552 @default.
• W1998045383 cites W2064931749 @default.
• W1998045383 cites W2066041565 @default.
• W1998045383 cites W2067714785 @default.
• W1998045383 cites W2072571320 @default.
• W1998045383 cites W2075483304 @default.
• W1998045383 cites W2083516462 @default.
• W1998045383 cites W2093878302 @default.
• W1998045383 cites W2097552134 @default.
• W1998045383 cites W2098533425 @default.
• W1998045383 cites W2105323399 @default.
• W1998045383 cites W2106043217 @default.
• W1998045383 cites W2110113231 @default.
• W1998045383 cites W2114320376 @default.
• W1998045383 cites W2115005877 @default.
• W1998045383 cites W2124597092 @default.
• W1998045383 cites W2133294519 @default.
• W1998045383 cites W2137410262 @default.
• W1998045383 cites W2149756514 @default.
• W1998045383 cites W2154962270 @default.
• W1998045383 cites W2160134999 @default.
• W1998045383 cites W2162166182 @default.
• W1998045383 cites W2573229794 @default.
• W1998045383 doi "https://doi.org/10.1073/pnas.0607097104" @default.
• W1998045383 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1785253" @default.
• W1998045383 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17251351" @default.
• W1998045383 hasPublicationYear "2007" @default.
• W1998045383 type Work @default.
• W1998045383 sameAs 1998045383 @default.
• W1998045383 citedByCount "332" @default.
• W1998045383 countsByYear W19980453832012 @default.
• W1998045383 countsByYear W19980453832013 @default.
• W1998045383 countsByYear W19980453832014 @default.
• W1998045383 countsByYear W19980453832015 @default.
• W1998045383 countsByYear W19980453832016 @default.
• W1998045383 countsByYear W19980453832017 @default.
• W1998045383 countsByYear W19980453832018 @default.
• W1998045383 countsByYear W19980453832019 @default.
• W1998045383 countsByYear W19980453832020 @default.
• W1998045383 countsByYear W19980453832021 @default.
• W1998045383 countsByYear W19980453832022 @default.
• W1998045383 countsByYear W19980453832023 @default.
• W1998045383 crossrefType "journal-article" @default.
• W1998045383 hasAuthorship W1998045383A5032949743 @default.
• W1998045383 hasAuthorship W1998045383A5059620610 @default.
• W1998045383 hasAuthorship W1998045383A5066209297 @default.
• W1998045383 hasAuthorship W1998045383A5074112300 @default.
• W1998045383 hasAuthorship W1998045383A5075318212 @default.
• W1998045383 hasBestOaLocation W19980453832 @default.
• W1998045383 hasConcept C11268172 @default.
• W1998045383 hasConcept C113196181 @default.
• W1998045383 hasConcept C121332964 @default.
• W1998045383 hasConcept C121864883 @default.
• W1998045383 hasConcept C12554922 @default.
• W1998045383 hasConcept C147597530 @default.
• W1998045383 hasConcept C147789679 @default.
• W1998045383 hasConcept C159467904 @default.
• W1998045383 hasConcept C168568712 @default.
• W1998045383 hasConcept C178790620 @default.
• W1998045383 hasConcept C181199279 @default.
• W1998045383 hasConcept C184651966 @default.
• W1998045383 hasConcept C185592680 @default.
• W1998045383 hasConcept C204328495 @default.
• W1998045383 hasConcept C207374878 @default.
• W1998045383 hasConcept C2777654166 @default.
• W1998045383 hasConcept C2780004032 @default.
• W1998045383 hasConcept C2781455991 @default.

• next