Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000589064> ?p ?o ?g. }
- W2000589064 abstract "Splenic transitional B-cells (T1 and T2) are selected to avoid self-reactivity and to safeguard against autoimmunity, then differentiate into mature follicular (FO-I and FO-II) and marginal zone (MZ) subsets. Transcriptomic analysis by RNA-seq of the five B-cell subsets revealed T1 cell signature genes included RAG suggesting a potential for receptor revision. T1 to T2 B-cell differentiation was marked by a switch from Myb to Myc, increased expression of the PI3K adapter DAP10 and MHC class II. FO-II may be an intermediate in FO-I differentiation and may also become MZ B-cells as suggested by principle component analysis. MZ B-cells possessed the most distinct transcriptome including down-regulation of CD45 phosphatase-associated protein (CD45-AP/PTPRC-AP), as well as upregulation of IL-9R and innate molecules TLR3, TLR7, and bactericidal Perforin-2 (MPEG1). Among the endosomal TLRs, stimulation via TLR3 further enhanced Perforin-2 expression exclusively in MZ B-cells. Using gene-deleted and overexpressing transgenic mice we show that IL-9/IL-9R interaction resulted in rapid activation of STAT1, 3, and 5, primarily in MZ B-cells. Importantly, CD45-AP mutant mice had reduced transitional and increased mature MZ and FO B-cells, suggesting that it prevents premature entry of transitional B-cells to the mature B-cell pool or their survival and proliferation. Together, these findings suggest, developmental plasticity among splenic B-cell subsets, potential for receptor revision in peripheral tolerance whereas enhanced metabolism coincides with T2 to mature B-cell differentiation. Further, unique core transcriptional signatures in MZ B-cells may control their innate features." @default.
- W2000589064 created "2016-06-24" @default.
- W2000589064 creator A5016967871 @default.
- W2000589064 creator A5021028530 @default.
- W2000589064 creator A5023050141 @default.
- W2000589064 creator A5023066544 @default.
- W2000589064 creator A5042245279 @default.
- W2000589064 creator A5062966701 @default.
- W2000589064 creator A5064301049 @default.
- W2000589064 creator A5066632557 @default.
- W2000589064 creator A5068755244 @default.
- W2000589064 creator A5076315131 @default.
- W2000589064 creator A5077734866 @default.
- W2000589064 creator A5083114133 @default.
- W2000589064 creator A5087254915 @default.
- W2000589064 date "2015-02-11" @default.
- W2000589064 modified "2023-10-16" @default.
- W2000589064 title "Distinct Transcriptomic Features are Associated with Transitional and Mature B-Cell Populations in the Mouse Spleen" @default.
- W2000589064 cites W1483118877 @default.
- W2000589064 cites W1484787354 @default.
- W2000589064 cites W1505026047 @default.
- W2000589064 cites W1565742957 @default.
- W2000589064 cites W1595532902 @default.
- W2000589064 cites W1598152151 @default.
- W2000589064 cites W1783364792 @default.
- W2000589064 cites W1789348297 @default.
- W2000589064 cites W1826755587 @default.
- W2000589064 cites W1840301514 @default.
- W2000589064 cites W1918361100 @default.
- W2000589064 cites W1937071194 @default.
- W2000589064 cites W1942882022 @default.
- W2000589064 cites W1975225648 @default.
- W2000589064 cites W1975507673 @default.
- W2000589064 cites W1977099733 @default.
- W2000589064 cites W1980583475 @default.
- W2000589064 cites W1982460924 @default.
- W2000589064 cites W1986088199 @default.
- W2000589064 cites W1986401073 @default.
- W2000589064 cites W1986644831 @default.
- W2000589064 cites W1986730787 @default.
- W2000589064 cites W1987167729 @default.
- W2000589064 cites W1987529064 @default.
- W2000589064 cites W1991742833 @default.
- W2000589064 cites W1993738774 @default.
- W2000589064 cites W2000111555 @default.
- W2000589064 cites W2002229146 @default.
- W2000589064 cites W2005862926 @default.
- W2000589064 cites W2009603041 @default.
- W2000589064 cites W2009641021 @default.
- W2000589064 cites W2011924190 @default.
- W2000589064 cites W2018196963 @default.
- W2000589064 cites W2019213477 @default.
- W2000589064 cites W2020259260 @default.
- W2000589064 cites W2021133590 @default.
- W2000589064 cites W2024640284 @default.
- W2000589064 cites W2028029079 @default.
- W2000589064 cites W2030513906 @default.
- W2000589064 cites W2032286150 @default.
- W2000589064 cites W2036404541 @default.
- W2000589064 cites W2038264470 @default.
- W2000589064 cites W2038664895 @default.
- W2000589064 cites W2038729399 @default.
- W2000589064 cites W2040803520 @default.
- W2000589064 cites W2046037953 @default.
- W2000589064 cites W2050288546 @default.
- W2000589064 cites W2052291773 @default.
- W2000589064 cites W2060368420 @default.
- W2000589064 cites W2062172468 @default.
- W2000589064 cites W2064223130 @default.
- W2000589064 cites W2066974259 @default.
- W2000589064 cites W2068661921 @default.
- W2000589064 cites W2071165514 @default.
- W2000589064 cites W2079232338 @default.
- W2000589064 cites W2079999658 @default.
- W2000589064 cites W2082416335 @default.
- W2000589064 cites W2083945266 @default.
- W2000589064 cites W2090316299 @default.
- W2000589064 cites W2092896429 @default.
- W2000589064 cites W2093542167 @default.
- W2000589064 cites W2098673687 @default.
- W2000589064 cites W2100305481 @default.
- W2000589064 cites W2100434800 @default.
- W2000589064 cites W2101458514 @default.
- W2000589064 cites W2101490791 @default.
- W2000589064 cites W2102771576 @default.
- W2000589064 cites W2105841943 @default.
- W2000589064 cites W2106804464 @default.
- W2000589064 cites W2107964063 @default.
- W2000589064 cites W2108188994 @default.
- W2000589064 cites W2110709185 @default.
- W2000589064 cites W2124343538 @default.
- W2000589064 cites W2124400367 @default.
- W2000589064 cites W2133465414 @default.
- W2000589064 cites W2134819036 @default.
- W2000589064 cites W2135169909 @default.
- W2000589064 cites W2138302399 @default.
- W2000589064 cites W2138483765 @default.
- W2000589064 cites W2138684248 @default.
- W2000589064 cites W2138706182 @default.
- W2000589064 cites W2140036340 @default.