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- W2010374730 abstract "ObjectiveTo examine the effect of oestrogen depletion produced by surgical ovariectomy on the structural and biomechanical properties of ovine femoro-tibial articular cartilage (AC), and the production of inducible nitric oxide synthase (iNOS) and nitrotyrosine by these tissues.MethodsSix aged ewes were surgically ovariectomised (OVX), while six were used as unoperated controls. Dynamic biomechanical indentation testing of tibial plateau AC was performed at 26 weeks post-op. Histological sections of medial tibial plateau and lateral tibial plateau (LTP), medial and lateral femoral condyles (MFC, LFC) and patellar AC were examined for histopathology, toluidine blue staining intensity, and patterns of collagen birefringence intensity. Immunoreactivity for iNOS and nitrotyrosine was assessed in full-thickness biopsy plugs of LFC and patellar AC, and patellar AC explants were cultured to determine in vitro NO release.ResultsPhase lag was reduced overall in LTP-AC of OVX sheep (10.9±2.2° vs 12.1±2.3°; P<0.0001). Cartilage thickness was reduced in the LTP of OVX sheep (P=0.0002), in association with localised changes in dynamic shear modulus. Toluidine blue staining intensity was reduced in the patella, LFC, and MFC. Histological examination revealed greater histopathology scores in the MFC of OVX animals, and altered collagen birefringence intensity plots in the LTP. Immunostaining for iNOS was increased in patella AC (P=0.008), whilst nitrotyrosine immunoreactivity was increased in patella (P=0.03) and LFC (P<0.0001) AC. NO release by patellar AC explants was also elevated.ConclusionsOestrogen depletion induced by OVX caused regional thinning of femoro-tibial cartilage, with biomechanical and histological changes suggestive of a disturbance in the content and/or structural organisation of the proteoglycan and collagen macromolecular assembly. The observed up-regulation of cartilage iNOS suggests a possible mechanism for these matrix changes." @default.
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- W2010374730 date "2005-12-01" @default.
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- W2010374730 title "Ovariectomy alters the structural and biomechanical properties of ovine femoro-tibial articular cartilage and increases cartilage iNOS" @default.
- W2010374730 cites W123183995 @default.
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- W2010374730 cites W1972426221 @default.
- W2010374730 cites W1978552679 @default.
- W2010374730 cites W1979837737 @default.
- W2010374730 cites W1981923756 @default.
- W2010374730 cites W1990802251 @default.
- W2010374730 cites W1991040567 @default.
- W2010374730 cites W1992335976 @default.
- W2010374730 cites W1993840291 @default.
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- W2010374730 cites W2005150459 @default.
- W2010374730 cites W2008413355 @default.
- W2010374730 cites W2009528443 @default.
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- W2010374730 cites W2031736769 @default.
- W2010374730 cites W2035469813 @default.
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- W2010374730 cites W2053293951 @default.
- W2010374730 cites W2057526133 @default.
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- W2010374730 cites W2063580118 @default.
- W2010374730 cites W2066301620 @default.
- W2010374730 cites W2066659578 @default.
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- W2010374730 cites W2070288401 @default.
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- W2010374730 cites W2102513693 @default.
- W2010374730 cites W2103626890 @default.
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- W2010374730 doi "https://doi.org/10.1016/j.joca.2005.07.001" @default.
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