FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2013804512> ?p ?o ?g. }

• W2013804512 endingPage "41" @default.
• W2013804512 startingPage "31" @default.
• W2013804512 abstract "Abstract Barrier-to-autointegration factor (BAF) is a conserved 10 kDa DNA-binding protein. BAF interacts with LEM-domain proteins including emerin, LAP2β, and MAN1 in the inner nuclear membrane. Using fluorescence recovery after photobleaching (FRAP) and fluorescence loss in photobleaching (FLIP), we compared the mobility of BAF to its partners emerin, LAP2β, and MAN1 in living HeLa cells. Like endogenous BAF, GFP–BAF was enriched at the nuclear envelope, and found inside the nucleus and in the cytoplasm during interphase. At every location, FRAP and FLIP analysis showed that GFP–BAF diffused rapidly; the halftimes for recovery in a 0.8 μm square area were 260 ms at the nuclear envelope, and even faster inside the nucleus and in the cytoplasm. GFP-fused emerin, LAP2β, and MAN1 were all relatively immobile, with recovery halftimes of about 1 min, for a 2 μm square area. Thus, BAF is dynamic and mobile during interphase, in stark contrast to its nuclear envelope partners. FLIP results further showed that rapidly diffusing cytoplasmic and nuclear pools of GFP–BAF were distinctly regulated, with nuclear GFP–BAF unable to replenish cytoplasmic BAF. Fluorescence resonance energy transfer (FRET) results showed that CFP–BAF binds directly to YFP–emerin at the inner nuclear membrane of living cells. We propose a “touch-and-go” model in which BAF binds emerin frequently but transiently during interphase. These findings contrast with the slow mobility of both GFP–BAF and GFP–emerin during telophase, when they colocalized at the ‘core’ region of telophase chromosomes at early stages of nuclear assembly." @default.
• W2013804512 created "2016-06-24" @default.
• W2013804512 creator A5038535500 @default.
• W2013804512 creator A5065796724 @default.
• W2013804512 creator A5068285332 @default.
• W2013804512 creator A5076833397 @default.
• W2013804512 creator A5083599087 @default.
• W2013804512 creator A5086047056 @default.
• W2013804512 date "2004-07-01" @default.
• W2013804512 modified "2023-10-01" @default.
• W2013804512 title "Dynamic interaction between BAF and emerin revealed by FRAP, FLIP, and FRET analyses in living HeLa cells" @default.
• W2013804512 cites W1530729226 @default.
• W2013804512 cites W1597994956 @default.
• W2013804512 cites W1833934291 @default.
• W2013804512 cites W1845088912 @default.
• W2013804512 cites W1888731578 @default.
• W2013804512 cites W1941933392 @default.
• W2013804512 cites W1950573144 @default.
• W2013804512 cites W1965965755 @default.
• W2013804512 cites W1966733909 @default.
• W2013804512 cites W1973779048 @default.
• W2013804512 cites W1984675480 @default.
• W2013804512 cites W1985349579 @default.
• W2013804512 cites W2010054905 @default.
• W2013804512 cites W2012502527 @default.
• W2013804512 cites W2017934591 @default.
• W2013804512 cites W2025286269 @default.
• W2013804512 cites W2030316404 @default.
• W2013804512 cites W2034525269 @default.
• W2013804512 cites W2034844603 @default.
• W2013804512 cites W2040059091 @default.
• W2013804512 cites W2040257217 @default.
• W2013804512 cites W2041957522 @default.
• W2013804512 cites W2046252690 @default.
• W2013804512 cites W2056204193 @default.
• W2013804512 cites W2060489446 @default.
• W2013804512 cites W2092987144 @default.
• W2013804512 cites W2108130094 @default.
• W2013804512 cites W2111013687 @default.
• W2013804512 cites W2120957782 @default.
• W2013804512 cites W2136583609 @default.
• W2013804512 cites W2142891272 @default.
• W2013804512 cites W2143351999 @default.
• W2013804512 cites W2145893833 @default.
• W2013804512 cites W2149823296 @default.
• W2013804512 cites W2152214759 @default.
• W2013804512 cites W2156538971 @default.
• W2013804512 cites W2163400869 @default.
• W2013804512 cites W2165187823 @default.
• W2013804512 cites W2167403012 @default.
• W2013804512 cites W2171721764 @default.
• W2013804512 cites W2209964471 @default.
• W2013804512 doi "https://doi.org/10.1016/j.jsb.2003.11.013" @default.
• W2013804512 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15109603" @default.
• W2013804512 hasPublicationYear "2004" @default.
• W2013804512 type Work @default.
• W2013804512 sameAs 2013804512 @default.
• W2013804512 citedByCount "130" @default.
• W2013804512 countsByYear W20138045122012 @default.
• W2013804512 countsByYear W20138045122013 @default.
• W2013804512 countsByYear W20138045122014 @default.
• W2013804512 countsByYear W20138045122015 @default.
• W2013804512 countsByYear W20138045122016 @default.
• W2013804512 countsByYear W20138045122017 @default.
• W2013804512 countsByYear W20138045122018 @default.
• W2013804512 countsByYear W20138045122019 @default.
• W2013804512 countsByYear W20138045122020 @default.
• W2013804512 countsByYear W20138045122021 @default.
• W2013804512 countsByYear W20138045122022 @default.
• W2013804512 countsByYear W20138045122023 @default.
• W2013804512 crossrefType "journal-article" @default.
• W2013804512 hasAuthorship W2013804512A5038535500 @default.
• W2013804512 hasAuthorship W2013804512A5065796724 @default.
• W2013804512 hasAuthorship W2013804512A5068285332 @default.
• W2013804512 hasAuthorship W2013804512A5076833397 @default.
• W2013804512 hasAuthorship W2013804512A5083599087 @default.
• W2013804512 hasAuthorship W2013804512A5086047056 @default.
• W2013804512 hasConcept C104317684 @default.
• W2013804512 hasConcept C120665830 @default.
• W2013804512 hasConcept C121332964 @default.
• W2013804512 hasConcept C12554922 @default.
• W2013804512 hasConcept C1491633281 @default.
• W2013804512 hasConcept C185592680 @default.
• W2013804512 hasConcept C190283241 @default.
• W2013804512 hasConcept C2776591724 @default.
• W2013804512 hasConcept C2777366897 @default.
• W2013804512 hasConcept C2781023310 @default.
• W2013804512 hasConcept C29512474 @default.
• W2013804512 hasConcept C54355233 @default.
• W2013804512 hasConcept C86339819 @default.
• W2013804512 hasConcept C86803240 @default.
• W2013804512 hasConcept C91881484 @default.
• W2013804512 hasConcept C95444343 @default.
• W2013804512 hasConcept C96305047 @default.
• W2013804512 hasConceptScore W2013804512C104317684 @default.
• W2013804512 hasConceptScore W2013804512C120665830 @default.
• W2013804512 hasConceptScore W2013804512C121332964 @default.
• W2013804512 hasConceptScore W2013804512C12554922 @default.

• next