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• W2016292406 abstract "Gene expression analysis by reverse transcriptase real-time or quantitative polymerase chain reaction (RT-qPCR) is becoming widely used for non-model plant species. Given the high sensitivity of this method, normalization using multiple housekeeping or reference genes is critical, and careful selection of these reference genes is one of the most important steps to obtain reliable results. In this study, reference genes commonly used for other plant species were investigated to identify genes displaying highly uniform expression patterns in different varieties, tissues, developmental stages, fungal infection, and osmotic stress conditions for the non-model crop Musa (banana and plantains). The expression stability of six candidate reference genes was tested on six different sample sets, and the results were analyzed using the publicly available algorithms geNorm and NormFinder. Our results show that variety, plant material, primer set, and gene identity can all influence the robustness and outcome of RT-qPCR analysis. In the case of Musa, a combination of three reference genes (EF1, TUB and ACT) can be used for normalization of gene expression data from greenhouse leaf samples. In the case of shoot meristem cultures, numerous combinations can be used because the investigated reference genes exhibited limited variability. In contrast, variability in expression of the reference genes was much larger among leaf samples from plants grown in vitro, for which the best combination of reference genes (L2 and ACT genes) is still suboptimal. Overall, our data confirm that the stability of candidate reference genes should be thoroughly investigated for each experimental condition under investigation." @default.
• W2016292406 created "2016-06-24" @default.
• W2016292406 creator A5026310692 @default.
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• W2016292406 creator A5058002183 @default.
• W2016292406 creator A5080700128 @default.
• W2016292406 creator A5089013289 @default.
• W2016292406 date "2012-06-08" @default.
• W2016292406 modified "2023-09-26" @default.
• W2016292406 title "Selection and validation of reference genes for quantitative RT-PCR expression studies of the non-model crop Musa" @default.
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• W2016292406 doi "https://doi.org/10.1007/s11032-012-9711-1" @default.
• W2016292406 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3460175" @default.
• W2016292406 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23024595" @default.
• W2016292406 hasPublicationYear "2012" @default.
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