FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2019643669> ?p ?o ?g. }

• W2019643669 endingPage "404" @default.
• W2019643669 startingPage "397" @default.
• W2019643669 abstract "Prostaglandins are locally produced in a number of tissues in response to a variety of stimuli, including local growth factors and systemic hormones. The present investigation characterizes prostaglandin effects on growth plate chondrocytes. Since cyclic adenosine monophosphate (cAMP) may act as a prostaglandin-stimulated second messenger, the effects of prostaglandins A1, D2, E1, E2, F2 alpha, and I2 (10(-10)-10(-6) M) on cAMP levels and thymidine incorporation were evaluated. The stimulation of cAMP and thymidine incorporation by the various prostaglandin metabolites were dose dependent and highly correlated (r = 0.99, p less than 0.001). The magnitude of the effect varied but was maximal at 10(-6) M for each of the prostaglandins. Prostaglandins of the E series (E1 and E2) were the most potent, causing significant effects at 10(-10) M and with maximal 12- and 13-fold increases in DNA synthesis after a 24 h exposure. Prostaglandins D2 and A1 maximally stimulated thymidine incorporation by 4.7- and 3.1-fold but caused significant increases only at 10(-8) M. Prostaglandins F2 alpha and I2 were the least stimulatory, producing small but significant increases in thymidine incorporation at 10(-6) M (30 and 100% stimulations). A causal relationship between cAMP and thymidine incorporation was further verified by the ability of dibutyryl-cAMP to increase DNA synthesis. Long-term chondrocyte cultures treated continuously with PGE2 demonstrated an increase in cell number, confirming the proliferative effect. Indomethacin did not alter the potent dose-dependent stimulations of chondrocyte DNA synthesis by TGF-beta 1, basic FGF, or PTH, indicating that these known mitogens act independently of prostaglandin metabolism. PGE2 was further examined for its effects of matrix synthesis. PGE2 inhibited collagen synthesis with a maximal 42% decrease but did not alter noncollagen protein synthesis. In contrast, PGE2 maximally increased sulfate incorporation by 35% and caused a small dose-dependent inhibition in alkaline phosphatase activity. Thus, prostaglandins alter DNA and matrix synthesis in growth plate chondrocytes and may have an important role in chondrocyte metabolism in the growth plate, fracture callus, and other areas of endochondral ossification." @default.
• W2019643669 created "2016-06-24" @default.
• W2019643669 creator A5024783120 @default.
• W2019643669 creator A5027366738 @default.
• W2019643669 creator A5041375808 @default.
• W2019643669 creator A5059190704 @default.
• W2019643669 date "2009-12-03" @default.
• W2019643669 modified "2023-10-14" @default.
• W2019643669 title "Influence of prostaglandins on DNA and matrix synthesis in growth plate chondrocytes" @default.
• W2019643669 cites W1276805569 @default.
• W2019643669 cites W138983931 @default.
• W2019643669 cites W1989635205 @default.
• W2019643669 cites W1993944146 @default.
• W2019643669 cites W1996908950 @default.
• W2019643669 cites W2003239104 @default.
• W2019643669 cites W2003571415 @default.
• W2019643669 cites W2006880764 @default.
• W2019643669 cites W2009915497 @default.
• W2019643669 cites W2017603039 @default.
• W2019643669 cites W2030578204 @default.
• W2019643669 cites W2034639872 @default.
• W2019643669 cites W2037484179 @default.
• W2019643669 cites W2039386859 @default.
• W2019643669 cites W2040020097 @default.
• W2019643669 cites W2040771015 @default.
• W2019643669 cites W2058486668 @default.
• W2019643669 cites W2062026488 @default.
• W2019643669 cites W2062510144 @default.
• W2019643669 cites W2067986741 @default.
• W2019643669 cites W2075860965 @default.
• W2019643669 cites W2078414513 @default.
• W2019643669 cites W2081279185 @default.
• W2019643669 cites W2095237219 @default.
• W2019643669 cites W2149509974 @default.
• W2019643669 cites W2156910766 @default.
• W2019643669 cites W2160919847 @default.
• W2019643669 doi "https://doi.org/10.1002/jbmr.5650070407" @default.
• W2019643669 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1319104" @default.
• W2019643669 hasPublicationYear "2009" @default.
• W2019643669 type Work @default.
• W2019643669 sameAs 2019643669 @default.
• W2019643669 citedByCount "67" @default.
• W2019643669 countsByYear W20196436692013 @default.
• W2019643669 countsByYear W20196436692014 @default.
• W2019643669 countsByYear W20196436692015 @default.
• W2019643669 countsByYear W20196436692016 @default.
• W2019643669 countsByYear W20196436692018 @default.
• W2019643669 countsByYear W20196436692019 @default.
• W2019643669 countsByYear W20196436692020 @default.
• W2019643669 countsByYear W20196436692021 @default.
• W2019643669 countsByYear W20196436692022 @default.
• W2019643669 crossrefType "journal-article" @default.
• W2019643669 hasAuthorship W2019643669A5024783120 @default.
• W2019643669 hasAuthorship W2019643669A5027366738 @default.
• W2019643669 hasAuthorship W2019643669A5041375808 @default.
• W2019643669 hasAuthorship W2019643669A5059190704 @default.
• W2019643669 hasConcept C126322002 @default.
• W2019643669 hasConcept C134018914 @default.
• W2019643669 hasConcept C185592680 @default.
• W2019643669 hasConcept C202751555 @default.
• W2019643669 hasConcept C24998067 @default.
• W2019643669 hasConcept C2776991684 @default.
• W2019643669 hasConcept C2777108182 @default.
• W2019643669 hasConcept C2777198256 @default.
• W2019643669 hasConcept C2777956040 @default.
• W2019643669 hasConcept C2779837120 @default.
• W2019643669 hasConcept C2780664492 @default.
• W2019643669 hasConcept C2781403057 @default.
• W2019643669 hasConcept C552990157 @default.
• W2019643669 hasConcept C55493867 @default.
• W2019643669 hasConcept C62112901 @default.
• W2019643669 hasConcept C64241487 @default.
• W2019643669 hasConcept C71924100 @default.
• W2019643669 hasConcept C86803240 @default.
• W2019643669 hasConceptScore W2019643669C126322002 @default.
• W2019643669 hasConceptScore W2019643669C134018914 @default.
• W2019643669 hasConceptScore W2019643669C185592680 @default.
• W2019643669 hasConceptScore W2019643669C202751555 @default.
• W2019643669 hasConceptScore W2019643669C24998067 @default.
• W2019643669 hasConceptScore W2019643669C2776991684 @default.
• W2019643669 hasConceptScore W2019643669C2777108182 @default.
• W2019643669 hasConceptScore W2019643669C2777198256 @default.
• W2019643669 hasConceptScore W2019643669C2777956040 @default.
• W2019643669 hasConceptScore W2019643669C2779837120 @default.
• W2019643669 hasConceptScore W2019643669C2780664492 @default.
• W2019643669 hasConceptScore W2019643669C2781403057 @default.
• W2019643669 hasConceptScore W2019643669C552990157 @default.
• W2019643669 hasConceptScore W2019643669C55493867 @default.
• W2019643669 hasConceptScore W2019643669C62112901 @default.
• W2019643669 hasConceptScore W2019643669C64241487 @default.
• W2019643669 hasConceptScore W2019643669C71924100 @default.
• W2019643669 hasConceptScore W2019643669C86803240 @default.
• W2019643669 hasIssue "4" @default.
• W2019643669 hasLocation W20196436691 @default.
• W2019643669 hasLocation W20196436692 @default.
• W2019643669 hasOpenAccess W2019643669 @default.
• W2019643669 hasPrimaryLocation W20196436691 @default.
• W2019643669 hasRelatedWork W1605653259 @default.

• next