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• W2019923908 abstract "RNA silencing plays important roles in the regulation of gene expression in eukaryotes. We previously reported that RNA silencing of a linked endogenous gene and a transgene in transgenic rice seeds can be induced by the expression of foreign recombinant mGLP-1, which acts as a silencing-inducible sequence through RNA interference. In this study, we found that the induction of RNA silencing by foreign transgenes is not restricted to mGLP-1 but is observed in many other genes as a relatively general phenomenon, as several foreign genes were involved in inducing RNA silencing in the same manner as mGLP-1 in transgenic rice. We detected 21-24-nt siRNAs using both sense and antisense probes specific to the silenced genes in both the leaves and endosperm of transgenic rice plants. Moreover, read-through transcripts were consistently observed in silenced transgenic rice plants. Taken together, these results suggest that proper transcription termination was prevented in these plants, and the highly divergent 3'-end transcripts served as templates for double-stranded RNA synthesis, resulting in the degradation of the target genes via siRNA." @default.
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• W2019923908 date "2014-08-01" @default.
• W2019923908 modified "2023-10-16" @default.
• W2019923908 title "RNA silencing is induced by the expression of foreign recombinant products in transgenic rice" @default.
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• W2019923908 doi "https://doi.org/10.1016/j.plantsci.2014.06.008" @default.
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