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- W2019971107 abstract "The R1 restriction endonuclease from bacteria containing the RTF-1 plasmid cleaves polyoma virus DNA at a single site. Virus stocks which have been passaged through cells several times at moderate or high multiplicities contain closed circular DNAs which are not cleaved by the R1 endonuclease. One such population of DNAs, is not infectious, and when sheared and denatured, reassociates at a rate approximately four times faster than polyoma DNA. The predominant DNA in the population is 10% smaller than polyoma DNA and contains a fraction of the polyoma genome tandemly repeated three to four times. There is no significant contamination of the covalently closed circular DNAs by repetitive host sequences. A model explaining how such trandemly repeated molecules could arise by abortive rounds of polyoma DNA replication is discussed." @default.
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- W2019971107 date "1974-09-01" @default.
- W2019971107 modified "2023-09-26" @default.
- W2019971107 title "Closed circular DNAs with tandem repeats of a sequence from polyoma virus" @default.
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- W2019971107 doi "https://doi.org/10.1016/0042-6822(74)90249-9" @default.
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