FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2020593485> ?p ?o ?g. }

• W2020593485 endingPage "700" @default.
• W2020593485 startingPage "700" @default.
• W2020593485 abstract "Copper (Cu) is an essential biometal involved in a number of cell functions. Abnormal Cu homeostasis has been identified as a major factor in a number of neurodegenerative disorders. However, little is known about how cells of brain origin maintain Cu homeostasis and in particular, how they respond to an elevated Cu environment. Understanding these processes is essential to obtaining a greater insight into the pathological changes in neurodegeneration and ageing. Although previous studies have shown that Cu in neurons can be associated with synaptic function, there is little understanding of how Cu modulates the regulated secretory vesicle pathways in these cells. In this study, we examined the effect of elevated intracellular Cu on proteins associated with the regulated secretory vesicle pathway in NGF-differentiated PC12 cells that exhibit neuronal-like properties. Increasing intracellular Cu with a cell-permeable Cu-complex (CuII(gtsm)) resulted in increased expression of synaptophysin and robust translocation of this and additional vesicular proteins from synaptic-like microvesicle (SLMV) fractions to chromogranin-containing putative large dense core vesicle (LDCV) fractions in density gradient preparations. The LDCV fractions also contained substantially elevated Cu levels upon treatment of cells with CuII(gtsm). Expression of the H+ pump, V-ATPase, which is essential for vesicle maturation, was increased in Cu-treated cells while inhibition of V-ATPase prevented translocation of synaptophysin to LDCV fractions. Cu treatment was found to inhibit release of LDCVs in chromaffin cells due to reduced Ca2+-mediated vesicle exocytosis. Our findings demonstrate that elevated Cu can modulate LDCV metabolism potentially resulting in sequestration of Cu in this vesicle pool." @default.
• W2020593485 created "2016-06-24" @default.
• W2020593485 creator A5003193918 @default.
• W2020593485 creator A5004333747 @default.
• W2020593485 creator A5006373060 @default.
• W2020593485 creator A5007109931 @default.
• W2020593485 creator A5011179811 @default.
• W2020593485 creator A5026838566 @default.
• W2020593485 creator A5033891416 @default.
• W2020593485 creator A5035184195 @default.
• W2020593485 creator A5035952377 @default.
• W2020593485 creator A5037008294 @default.
• W2020593485 creator A5045915090 @default.
• W2020593485 creator A5046274365 @default.
• W2020593485 creator A5047674491 @default.
• W2020593485 creator A5047844198 @default.
• W2020593485 creator A5047909561 @default.
• W2020593485 creator A5068019235 @default.
• W2020593485 creator A5074059460 @default.
• W2020593485 creator A5082551254 @default.
• W2020593485 date "2013-01-01" @default.
• W2020593485 modified "2023-10-18" @default.
• W2020593485 title "Copper modulates the large dense core vesicle secretory pathway in PC12 cells" @default.
• W2020593485 cites W1514837446 @default.
• W2020593485 cites W1516914558 @default.
• W2020593485 cites W1517283793 @default.
• W2020593485 cites W1584279734 @default.
• W2020593485 cites W192250034 @default.
• W2020593485 cites W1963698191 @default.
• W2020593485 cites W1966521314 @default.
• W2020593485 cites W1967868572 @default.
• W2020593485 cites W1968531119 @default.
• W2020593485 cites W1970587959 @default.
• W2020593485 cites W1971968078 @default.
• W2020593485 cites W1983308305 @default.
• W2020593485 cites W1991039509 @default.
• W2020593485 cites W1996124148 @default.
• W2020593485 cites W2004618150 @default.
• W2020593485 cites W2006193105 @default.
• W2020593485 cites W2008226264 @default.
• W2020593485 cites W2008618177 @default.
• W2020593485 cites W2011635665 @default.
• W2020593485 cites W2014210332 @default.
• W2020593485 cites W2022560938 @default.
• W2020593485 cites W2022745281 @default.
• W2020593485 cites W2023207330 @default.
• W2020593485 cites W2024136850 @default.
• W2020593485 cites W202852754 @default.
• W2020593485 cites W2033067408 @default.
• W2020593485 cites W2039675334 @default.
• W2020593485 cites W2048498385 @default.
• W2020593485 cites W2051205942 @default.
• W2020593485 cites W2052607444 @default.
• W2020593485 cites W2058936552 @default.
• W2020593485 cites W2060044295 @default.
• W2020593485 cites W2070865778 @default.
• W2020593485 cites W2076234140 @default.
• W2020593485 cites W2076855966 @default.
• W2020593485 cites W2081394379 @default.
• W2020593485 cites W2081544621 @default.
• W2020593485 cites W2083500052 @default.
• W2020593485 cites W2088370582 @default.
• W2020593485 cites W2089281646 @default.
• W2020593485 cites W2091737333 @default.
• W2020593485 cites W2091856845 @default.
• W2020593485 cites W2100743758 @default.
• W2020593485 cites W2108379668 @default.
• W2020593485 cites W2113525215 @default.
• W2020593485 cites W2118749647 @default.
• W2020593485 cites W2119544072 @default.
• W2020593485 cites W2119783541 @default.
• W2020593485 cites W2125334454 @default.
• W2020593485 cites W2125891731 @default.
• W2020593485 cites W2127914798 @default.
• W2020593485 cites W2128115766 @default.
• W2020593485 cites W2128124856 @default.
• W2020593485 cites W2139063637 @default.
• W2020593485 cites W2143880121 @default.
• W2020593485 cites W2154001547 @default.
• W2020593485 cites W2157567077 @default.
• W2020593485 cites W2158901625 @default.
• W2020593485 cites W2162631186 @default.
• W2020593485 cites W2166992796 @default.
• W2020593485 cites W2168148059 @default.
• W2020593485 cites W2168452237 @default.
• W2020593485 cites W2169595581 @default.
• W2020593485 cites W2169755174 @default.
• W2020593485 cites W2328607238 @default.
• W2020593485 doi "https://doi.org/10.1039/c3mt20231c" @default.
• W2020593485 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23661118" @default.
• W2020593485 hasPublicationYear "2013" @default.
• W2020593485 type Work @default.
• W2020593485 sameAs 2020593485 @default.
• W2020593485 citedByCount "10" @default.
• W2020593485 countsByYear W20205934852013 @default.
• W2020593485 countsByYear W20205934852015 @default.
• W2020593485 countsByYear W20205934852019 @default.
• W2020593485 countsByYear W20205934852020 @default.

• next