FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2023642213> ?p ?o ?g. }

• W2023642213 endingPage "389" @default.
• W2023642213 startingPage "383" @default.
• W2023642213 abstract "We have reinvestigated the molecular weight and subunit composition of calf thymus ribonuclease H1. Earlier studies suggested a variety of molecular weights for the enzyme in the range of 64K-84K and reported that the enzyme either was a single polypeptide of 74 kDa or consisted of from two to four subunits in the range of 21-34 kDa. Although we too find bands in this lower molecular weight range in our highly purified preparations following SDS-PAGE, our data suggest that the native structure of RNase H1 is a dimer of 68-kDa subunits. The evidence includes the following: (1) Western blot analysis of fractions taken at various stages of the purification indicates that the predominant antigenic form of the enzyme in crude extracts has a molecular weight of 68K but that during purification in the absence of sufficient protease inhibitors a variety of lower molecular weight forms appear concomitant with the disappearance of the 68-kDa band. (2) Activity gel analysis of the highly purified enzyme prepared in the presence of a battery of protease inhibitors reveals that the 68-kDa band (as well as several bands of lower molecular weight) possesses RNase H activity. (3) The 68-kDa band recognized by Western blotting with anti-RNase H immune sera is not detected by using preimmune sera. Furthermore, when immune sera are used, a trace of a 140-150-kDa antigenic form can sometimes be detected, consistent with the existence of a dimeric form of the enzyme.(ABSTRACT TRUNCATED AT 250 WORDS)" @default.
• W2023642213 created "2016-06-24" @default.
• W2023642213 creator A5006303381 @default.
• W2023642213 creator A5016097091 @default.
• W2023642213 date "1990-01-16" @default.
• W2023642213 modified "2023-09-27" @default.
• W2023642213 title "On the molecular weight and subunit composition of calf thymus ribonuclease H1" @default.
• W2023642213 cites W1496778348 @default.
• W2023642213 cites W1545344378 @default.
• W2023642213 cites W1565432868 @default.
• W2023642213 cites W1592224950 @default.
• W2023642213 cites W1603762414 @default.
• W2023642213 cites W1611482599 @default.
• W2023642213 cites W1697373019 @default.
• W2023642213 cites W1745439545 @default.
• W2023642213 cites W1971970938 @default.
• W2023642213 cites W1986952087 @default.
• W2023642213 cites W2003481405 @default.
• W2023642213 cites W2009687088 @default.
• W2023642213 cites W2019068034 @default.
• W2023642213 cites W2020476738 @default.
• W2023642213 cites W2034091109 @default.
• W2023642213 cites W2039960101 @default.
• W2023642213 cites W2054842101 @default.
• W2023642213 cites W2057639010 @default.
• W2023642213 cites W2057648499 @default.
• W2023642213 cites W2058052624 @default.
• W2023642213 cites W2064674376 @default.
• W2023642213 cites W2067343931 @default.
• W2023642213 cites W2080274676 @default.
• W2023642213 cites W2086449677 @default.
• W2023642213 cites W2100837269 @default.
• W2023642213 cites W2116506800 @default.
• W2023642213 cites W2128635872 @default.
• W2023642213 cites W2800298984 @default.
• W2023642213 doi "https://doi.org/10.1021/bi00454a012" @default.
• W2023642213 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2154245" @default.
• W2023642213 hasPublicationYear "1990" @default.
• W2023642213 type Work @default.
• W2023642213 sameAs 2023642213 @default.
• W2023642213 citedByCount "39" @default.
• W2023642213 countsByYear W20236422132012 @default.
• W2023642213 countsByYear W20236422132013 @default.
• W2023642213 countsByYear W20236422132014 @default.
• W2023642213 countsByYear W20236422132015 @default.
• W2023642213 countsByYear W20236422132016 @default.
• W2023642213 crossrefType "journal-article" @default.
• W2023642213 hasAuthorship W2023642213A5006303381 @default.
• W2023642213 hasAuthorship W2023642213A5016097091 @default.
• W2023642213 hasConcept C104292427 @default.
• W2023642213 hasConcept C104317684 @default.
• W2023642213 hasConcept C10879258 @default.
• W2023642213 hasConcept C147483822 @default.
• W2023642213 hasConcept C153911025 @default.
• W2023642213 hasConcept C178790620 @default.
• W2023642213 hasConcept C181199279 @default.
• W2023642213 hasConcept C185592680 @default.
• W2023642213 hasConcept C2776415932 @default.
• W2023642213 hasConcept C2776714187 @default.
• W2023642213 hasConcept C2778976237 @default.
• W2023642213 hasConcept C2779546866 @default.
• W2023642213 hasConcept C54355233 @default.
• W2023642213 hasConcept C55493867 @default.
• W2023642213 hasConcept C58121356 @default.
• W2023642213 hasConcept C67705224 @default.
• W2023642213 hasConcept C85265743 @default.
• W2023642213 hasConcept C86803240 @default.
• W2023642213 hasConceptScore W2023642213C104292427 @default.
• W2023642213 hasConceptScore W2023642213C104317684 @default.
• W2023642213 hasConceptScore W2023642213C10879258 @default.
• W2023642213 hasConceptScore W2023642213C147483822 @default.
• W2023642213 hasConceptScore W2023642213C153911025 @default.
• W2023642213 hasConceptScore W2023642213C178790620 @default.
• W2023642213 hasConceptScore W2023642213C181199279 @default.
• W2023642213 hasConceptScore W2023642213C185592680 @default.
• W2023642213 hasConceptScore W2023642213C2776415932 @default.
• W2023642213 hasConceptScore W2023642213C2776714187 @default.
• W2023642213 hasConceptScore W2023642213C2778976237 @default.
• W2023642213 hasConceptScore W2023642213C2779546866 @default.
• W2023642213 hasConceptScore W2023642213C54355233 @default.
• W2023642213 hasConceptScore W2023642213C55493867 @default.
• W2023642213 hasConceptScore W2023642213C58121356 @default.
• W2023642213 hasConceptScore W2023642213C67705224 @default.
• W2023642213 hasConceptScore W2023642213C85265743 @default.
• W2023642213 hasConceptScore W2023642213C86803240 @default.
• W2023642213 hasIssue "2" @default.
• W2023642213 hasLocation W20236422131 @default.
• W2023642213 hasLocation W20236422132 @default.
• W2023642213 hasOpenAccess W2023642213 @default.
• W2023642213 hasPrimaryLocation W20236422131 @default.
• W2023642213 hasRelatedWork W109123086 @default.
• W2023642213 hasRelatedWork W1969451414 @default.
• W2023642213 hasRelatedWork W1981022833 @default.
• W2023642213 hasRelatedWork W1987454130 @default.
• W2023642213 hasRelatedWork W2088776290 @default.
• W2023642213 hasRelatedWork W2089975440 @default.
• W2023642213 hasRelatedWork W2184845675 @default.
• W2023642213 hasRelatedWork W2977673320 @default.

• next