FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2025509968> ?p ?o ?g. }

• W2025509968 endingPage "952" @default.
• W2025509968 startingPage "937" @default.
• W2025509968 abstract "We developed a simple, rapid, inexpensive, and highly sensitive and specific strategy for the detection and lineage differentiation of primate lentiviruses (PIV-ELISA). It is based on the use of two indirect ELISA methods using synthetic peptides mapping the gp41/36 region (detection component) and the V3 region (differentiation component) of four lentivirus lineages, namely SIVcpz/HIV-1 (groups M, O, N, and SIVcpz-gab), SIVmnd, SIVagm, and SIVsm/SIVmac/HIV-2. This strategy was evaluated with panels of sera originating from both humans and nonhuman primates. The human reference panel consisted of 144 HIV Western blot (WB)-positive sera in which the corresponding virus had been genotyped (HIV-1: 72 group M, 28 group O, and 6 group N; HIV-2: 21 subtype A and 10 subtype B; and 7 HIV-1+2) and 105 HIV WB-negative samples. The nonhuman primate reference panel consisted of 24 sera from monkeys infected by viruses belonging to the four lineages included in the PIV-ELISA strategy (5 chimpanzees, 5 macaques, 8 mandrills, and 6 vervets) and 42 samples from seronegative animals. Additional field evaluation panels consisted of 815 human sera from Gabon, Cameroon, and France and 537 samples from 25 nonhuman primate species. All the samples from the two reference panels were correctly detected and discriminated by PIV-ELISA. In the human field evaluation panel, the gp41/36 component correctly identified all the test samples, with 98% specificity. The V3 component discriminated 206 HIV-1 group M, 98 group O, 12 group M+O, and 128 HIV-2 sera. In the primate field evaluation panel, both gp41/36 and V3 detected and discriminated all the WB-positive samples originating from monkeys infected with SIVcpz, SIVagm-ver, SIVmnd-1, SIVmnd-2, SIVdrl, or SIVsun. These results were confirmed by genotyping in every case. Four SIV-infected red-capped mangabeys (confirmed by PCR) were correctly identified by gp41/36, but only two reacted with the V3 peptides in the absence of a specific SIVrcm V3 peptide. Addition of a V3 SIVrcm peptide discriminated all the SIVrcm-positive samples. Fourteen Papio papio samples were positive for SIVsm gp 36 and by WB, but negative by PCR, whereas three Papio cynocephalus samples were positive by gp41/36 but indeterminate by WB and negative by PCR. This combined ELISA system is thus highly sensitive and specific for antibodies directed against HIV and SIV. In addition, the V3-based serotyping results always agreed with genotyping results. This method should prove useful for studies of lentivirus prevalence and diversity in human and nonhuman primates, and may also have the potential to detect previously undescribed SIVs." @default.
• W2025509968 created "2016-06-24" @default.
• W2025509968 creator A5001404007 @default.
• W2025509968 creator A5002744107 @default.
• W2025509968 creator A5019370510 @default.
• W2025509968 creator A5021331083 @default.
• W2025509968 creator A5033674140 @default.
• W2025509968 creator A5035689477 @default.
• W2025509968 creator A5037084499 @default.
• W2025509968 creator A5048892255 @default.
• W2025509968 creator A5053204631 @default.
• W2025509968 creator A5057851296 @default.
• W2025509968 creator A5059019919 @default.
• W2025509968 creator A5067441583 @default.
• W2025509968 creator A5067832449 @default.
• W2025509968 creator A5071231772 @default.
• W2025509968 creator A5076070228 @default.
• W2025509968 creator A5077809855 @default.
• W2025509968 creator A5083071986 @default.
• W2025509968 date "2001-07-15" @default.
• W2025509968 modified "2023-10-16" @default.
• W2025509968 title "Synthetic Peptide Strategy for the Detection of and Discrimination among Highly Divergent Primate Lentiviruses" @default.
• W2025509968 cites W1532002686 @default.
• W2025509968 cites W1532308826 @default.
• W2025509968 cites W1543894591 @default.
• W2025509968 cites W1564811180 @default.
• W2025509968 cites W1574216409 @default.
• W2025509968 cites W1599245000 @default.
• W2025509968 cites W1659932244 @default.
• W2025509968 cites W1764288588 @default.
• W2025509968 cites W1810629547 @default.
• W2025509968 cites W1909395851 @default.
• W2025509968 cites W1952459954 @default.
• W2025509968 cites W1968066796 @default.
• W2025509968 cites W1973001511 @default.
• W2025509968 cites W1976970819 @default.
• W2025509968 cites W1981847755 @default.
• W2025509968 cites W1994485839 @default.
• W2025509968 cites W1999532445 @default.
• W2025509968 cites W2009031499 @default.
• W2025509968 cites W2009703950 @default.
• W2025509968 cites W2011259035 @default.
• W2025509968 cites W2021906926 @default.
• W2025509968 cites W2027968393 @default.
• W2025509968 cites W2030482575 @default.
• W2025509968 cites W2030604856 @default.
• W2025509968 cites W2031656441 @default.
• W2025509968 cites W2034858284 @default.
• W2025509968 cites W2036459277 @default.
• W2025509968 cites W2041052804 @default.
• W2025509968 cites W2041775837 @default.
• W2025509968 cites W2044478352 @default.
• W2025509968 cites W2045043344 @default.
• W2025509968 cites W2055669624 @default.
• W2025509968 cites W2056473717 @default.
• W2025509968 cites W2061114508 @default.
• W2025509968 cites W2073771240 @default.
• W2025509968 cites W2079437045 @default.
• W2025509968 cites W2081006952 @default.
• W2025509968 cites W2082128975 @default.
• W2025509968 cites W2089372703 @default.
• W2025509968 cites W2089384420 @default.
• W2025509968 cites W2092012373 @default.
• W2025509968 cites W2099144243 @default.
• W2025509968 cites W2102378887 @default.
• W2025509968 cites W2110538282 @default.
• W2025509968 cites W2111143795 @default.
• W2025509968 cites W2112638992 @default.
• W2025509968 cites W2113851986 @default.
• W2025509968 cites W2119056227 @default.
• W2025509968 cites W2119480559 @default.
• W2025509968 cites W2120994977 @default.
• W2025509968 cites W2123364760 @default.
• W2025509968 cites W2127164893 @default.
• W2025509968 cites W2129954747 @default.
• W2025509968 cites W2138462482 @default.
• W2025509968 cites W2142916238 @default.
• W2025509968 cites W2143473353 @default.
• W2025509968 cites W2154963941 @default.
• W2025509968 cites W2155621927 @default.
• W2025509968 cites W2157713905 @default.
• W2025509968 cites W2165895151 @default.
• W2025509968 cites W2167651284 @default.
• W2025509968 cites W2173476119 @default.
• W2025509968 cites W2177796734 @default.
• W2025509968 cites W4247971816 @default.
• W2025509968 doi "https://doi.org/10.1089/088922201750290050" @default.
• W2025509968 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11461679" @default.
• W2025509968 hasPublicationYear "2001" @default.
• W2025509968 type Work @default.
• W2025509968 sameAs 2025509968 @default.
• W2025509968 citedByCount "88" @default.
• W2025509968 countsByYear W20255099682012 @default.
• W2025509968 countsByYear W20255099682013 @default.
• W2025509968 countsByYear W20255099682014 @default.
• W2025509968 countsByYear W20255099682015 @default.
• W2025509968 countsByYear W20255099682016 @default.
• W2025509968 countsByYear W20255099682017 @default.

• next