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• W2027235524 startingPage "130" @default.
• W2027235524 abstract "Possible clastogenic and aneugenic effects of pesticides on human lymphocytes at concentrations likely to be encountered in residential and occupational exposure were evaluated with (and without) the use of metabolic activation (S9). To get a better insight into the content of micronuclei (MN) and other chromatin instabilities, lymphocyte preparations were hybridized using pancentromeric DNA probes. Frequency of the MN, nuclear buds (NB) and nucleoplasmic bridges (NPB) in cultures treated with glyphosate slightly increased from 3.5 μg/ml onward. Presence of S9 significantly elevated cytome assay parameters only at 580 μg/ml. No concentration-related increase of centromere (C+) and DAPI signals (DAPI+) was observed for glyphosate treatment. Terbuthylazine treatment showed a dose dependent increase in the number of MN without S9 significant at 0.0008 μg/ml and higher. At concentration lower than 1/16 LD50 occurrence of C+MN was significantly elevated regardless of S9, but not dose related, and in the presence of S9 only NBs containing centromere signals were observed. Carbofuran treatment showed concentration-dependent increase in the number of MN. The frequency of C + MN was significant from 0.008 μg/ml onward regardless of S9. Results suggest that lower concentrations of glyphosate have no hazardous effects on DNA, while terbuthylazine and carbofuran revealed a predominant aneugenic potential." @default.
• W2027235524 created "2016-06-24" @default.
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• W2027235524 date "2009-09-01" @default.
• W2027235524 modified "2023-10-13" @default.
• W2027235524 title "Characterization of chromatin instabilities induced by glyphosate, terbuthylazine and carbofuran using cytome FISH assay" @default.
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• W2027235524 doi "https://doi.org/10.1016/j.toxlet.2009.05.012" @default.
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