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• W2033033039 abstract "Alkaline protease activity of a local soil isolate of Penicillium sp. for soy protein hydrolysis has been explored under solid substrate fermentation (SSF) conditions. The optimum assay parameters for alkaline protease activity of Penicillium sp. were: pH 9.0 (borate buffer, 0.1 M) and temperature, 45 °C. Pre-incubation of enzyme extract with Fe3+, Hg2+ and Cu2+ drastically inhibited the enzyme activity, whereas Ca2+, Mg2+ and Mn2+ marginally enhanced it. Penicillium sp. grown at 28 °C for 3 days on wheat bran supplemented with 25 mg soy protein g−1 bran, moistened with M-9 salt solution (1 ml g−1), inoculated with 1×1010 spores g−1 and extracted with 0.1 M borate buffer was found to yield a high titre of alkaline protease." @default.
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• W2033033039 date "2004-04-01" @default.
• W2033033039 modified "2023-10-05" @default.
• W2033033039 title "Production of alkaline protease by Penicillium sp. under SSF conditions and its application to soy protein hydrolysis" @default.
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• W2033033039 doi "https://doi.org/10.1016/s0032-9592(03)00212-7" @default.
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