FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2034257027> ?p ?o ?g. }

• W2034257027 endingPage "131" @default.
• W2034257027 startingPage "118" @default.
• W2034257027 abstract "Large-scale animal cell operation is costly both in terms of facilities and consumables. Hence developmental studies with animal cells normally start at laboratory scale, often using small stirred tanks. In order to better optimise cell performance, it is necessary to know the physical conditions under which the cells are grown. In this study a laboratory-scale vessel (2 dm3 working volume) with two large-bladed paddle impellers was characterised hydrodynamically. Three different aeration methods (surface, sparging and membrane aeration) were investigated and compared. Power input and oxygen transfer rates to culture medium were determined as a function of agitation and gas flow rates. Non-dimensional correlations were established for each case, which can be useful for scale-up purposes. The results obtained indicate that power input is quite dependent on the vessel accessories: for the same agitation rate, the maximum power is required for the membrane structure and the minimum for surface aeration, with the addition of the sparger leading to an intermediate situation. Predictions found in the literature can be used for simple vessels, but may not be applicable when accessories are added to the vessel structure; in such cases, the use of experimental relationships are required. Oxygen transfer rate was dependent on the aeration method and working conditions (agitation and gas flow rates), particularly for sparger aeration. Membrane aeration gave larger oxygen transfer but higher gas pressure and flow rates were required. Surface aeration was the least effective method, nevertheless requiring gas flow rates similar to those used for membrane aeration. The aeration method of choice depends upon the culture and work specificities: surface aeration is limited to small cell concentrations and low oxygen consumption rates. For higher cell concentrations and oxygen consumption rates, both membrane and sparger aeration methods can be applied: the use of the sparger is limited to cells that are not affected by the presence of bubbles or the addition of surfactants, whereas the membrane aeration basket should not be used when a hydrodynamically controlled stirred tank is required." @default.
• W2034257027 created "2016-06-24" @default.
• W2034257027 creator A5020278081 @default.
• W2034257027 creator A5021060711 @default.
• W2034257027 creator A5033758131 @default.
• W2034257027 creator A5056144458 @default.
• W2034257027 creator A5058073147 @default.
• W2034257027 creator A5081078003 @default.
• W2034257027 date "1995-02-01" @default.
• W2034257027 modified "2023-10-07" @default.
• W2034257027 title "Influence of power input and aeration method on mass transfer in a laboratory animal cell culture vessel" @default.
• W2034257027 cites W120350405 @default.
• W2034257027 cites W1483909917 @default.
• W2034257027 cites W1894684139 @default.
• W2034257027 cites W1963664325 @default.
• W2034257027 cites W1964426143 @default.
• W2034257027 cites W1965876945 @default.
• W2034257027 cites W1968865385 @default.
• W2034257027 cites W1978464842 @default.
• W2034257027 cites W1997913754 @default.
• W2034257027 cites W2000334360 @default.
• W2034257027 cites W2002201651 @default.
• W2034257027 cites W2020774582 @default.
• W2034257027 cites W2046048860 @default.
• W2034257027 cites W2048599687 @default.
• W2034257027 cites W2053072959 @default.
• W2034257027 cites W2060102337 @default.
• W2034257027 cites W2093536687 @default.
• W2034257027 cites W2104488124 @default.
• W2034257027 cites W2120518688 @default.
• W2034257027 cites W2147730867 @default.
• W2034257027 cites W2156146236 @default.
• W2034257027 cites W2164603846 @default.
• W2034257027 doi "https://doi.org/10.1002/jctb.280620203" @default.
• W2034257027 hasPublicationYear "1995" @default.
• W2034257027 type Work @default.
• W2034257027 sameAs 2034257027 @default.
• W2034257027 citedByCount "21" @default.
• W2034257027 countsByYear W20342570272012 @default.
• W2034257027 countsByYear W20342570272014 @default.
• W2034257027 countsByYear W20342570272020 @default.
• W2034257027 crossrefType "journal-article" @default.
• W2034257027 hasAuthorship W2034257027A5020278081 @default.
• W2034257027 hasAuthorship W2034257027A5021060711 @default.
• W2034257027 hasAuthorship W2034257027A5033758131 @default.
• W2034257027 hasAuthorship W2034257027A5056144458 @default.
• W2034257027 hasAuthorship W2034257027A5058073147 @default.
• W2034257027 hasAuthorship W2034257027A5081078003 @default.
• W2034257027 hasConcept C121332964 @default.
• W2034257027 hasConcept C127413603 @default.
• W2034257027 hasConcept C147789679 @default.
• W2034257027 hasConcept C170579384 @default.
• W2034257027 hasConcept C172120300 @default.
• W2034257027 hasConcept C185592680 @default.
• W2034257027 hasConcept C192562407 @default.
• W2034257027 hasConcept C20556612 @default.
• W2034257027 hasConcept C39432304 @default.
• W2034257027 hasConcept C43617362 @default.
• W2034257027 hasConcept C51038369 @default.
• W2034257027 hasConcept C528095902 @default.
• W2034257027 hasConcept C548081761 @default.
• W2034257027 hasConcept C57879066 @default.
• W2034257027 hasConcept C86922832 @default.
• W2034257027 hasConcept C87717796 @default.
• W2034257027 hasConcept C97355855 @default.
• W2034257027 hasConceptScore W2034257027C121332964 @default.
• W2034257027 hasConceptScore W2034257027C127413603 @default.
• W2034257027 hasConceptScore W2034257027C147789679 @default.
• W2034257027 hasConceptScore W2034257027C170579384 @default.
• W2034257027 hasConceptScore W2034257027C172120300 @default.
• W2034257027 hasConceptScore W2034257027C185592680 @default.
• W2034257027 hasConceptScore W2034257027C192562407 @default.
• W2034257027 hasConceptScore W2034257027C20556612 @default.
• W2034257027 hasConceptScore W2034257027C39432304 @default.
• W2034257027 hasConceptScore W2034257027C43617362 @default.
• W2034257027 hasConceptScore W2034257027C51038369 @default.
• W2034257027 hasConceptScore W2034257027C528095902 @default.
• W2034257027 hasConceptScore W2034257027C548081761 @default.
• W2034257027 hasConceptScore W2034257027C57879066 @default.
• W2034257027 hasConceptScore W2034257027C86922832 @default.
• W2034257027 hasConceptScore W2034257027C87717796 @default.
• W2034257027 hasConceptScore W2034257027C97355855 @default.
• W2034257027 hasIssue "2" @default.
• W2034257027 hasLocation W20342570271 @default.
• W2034257027 hasOpenAccess W2034257027 @default.
• W2034257027 hasPrimaryLocation W20342570271 @default.
• W2034257027 hasRelatedWork W1972724946 @default.
• W2034257027 hasRelatedWork W1983296421 @default.
• W2034257027 hasRelatedWork W2034257027 @default.
• W2034257027 hasRelatedWork W2044197868 @default.
• W2034257027 hasRelatedWork W2076689522 @default.
• W2034257027 hasRelatedWork W2091105885 @default.
• W2034257027 hasRelatedWork W2154823445 @default.
• W2034257027 hasRelatedWork W2537148879 @default.
• W2034257027 hasRelatedWork W2740560988 @default.
• W2034257027 hasRelatedWork W3208884237 @default.
• W2034257027 hasVolume "62" @default.
• W2034257027 isParatext "false" @default.

• next