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• W2034857771 endingPage "356" @default.
• W2034857771 startingPage "347" @default.
• W2034857771 abstract "We examined the expression of VCAM-1 and MAdCAM-1 after bone marrow transplantation (BMT). We also examined the influence of α4β7 integrin blockade on the homing of cells to the bone marrow and spleen. The expression of VCAM-1 and MAdCAM-1 by endothelial cells in the spleen and bone marrow was examined by immunoelectron microscopy using colloidal gold and was analyzed semiquantitatively. To examine the role of α4β7 integrin in donor cells, a homing assay was conducted following α4β7 integrin blockade in bone marrow-derived hematopoietic cells or spleen colony cells. Immediately after BMT, the expression of VCAM-1 and MAdCAM1 markedly decreased, but expression recovered significantly between 12 and 24 h after BMT. VCAM-1 recovered more acutely than MAdCAM-1 from 12 h onward. In the group transplanted with anti-α4β7 integrin antibody-treated bone marrow cells, the numbers of homing cells in the spleen and bone marrow were significantly decreased in an antibody dose-dependent manner. However, the number of homing cells was not different in either the spleen or bone marrow between anti-α4β7 integrin antibody treated and untreated spleen colony cells. It has been reported that α4β1 integrin and its receptor VCAM-1 play major roles in the homing of hematopoietic cells to bone marrow. Our study indicates the importance of MAdCAM-1 and its ligand, α4β7 integrin, in the homing of bone marrow-derived hematopoietic cells, but not spleen colony-derived cells, to both the spleen and bone marrow." @default.
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• W2034857771 date "2008-01-01" @default.
• W2034857771 modified "2023-10-03" @default.
• W2034857771 title "Role of MAdCAM-1 and Its Ligand on the Homing of Transplanted Hematopoietic Cells in Irradiated Mice" @default.
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• W2034857771 doi "https://doi.org/10.1538/expanim.57.347" @default.
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