FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2035314369> ?p ?o ?g. }

• W2035314369 endingPage "703" @default.
• W2035314369 startingPage "694" @default.
• W2035314369 abstract "Sucrose is a major carbon source for industrial bioethanol production by Saccharomyces cerevisiae. In yeasts, two modes of sucrose metabolism occur: (i) extracellular hydrolysis by invertase, followed by uptake and metabolism of glucose and fructose, and (ii) uptake via sucrose-proton symport followed by intracellular hydrolysis and metabolism. Although alternative start codons in the SUC2 gene enable synthesis of extracellular and intracellular invertase isoforms, sucrose hydrolysis in S. cerevisiae predominantly occurs extracellularly. In anaerobic cultures, intracellular hydrolysis theoretically enables a 9% higher ethanol yield than extracellular hydrolysis, due to energy costs of sucrose-proton symport. This prediction was tested by engineering the promoter and 5' coding sequences of SUC2, resulting in predominant (94%) cytosolic localization of invertase. In anaerobic sucrose-limited chemostats, this iSUC2-strain showed an only 4% increased ethanol yield and high residual sucrose concentrations indicated suboptimal sucrose-transport kinetics. To improve sucrose-uptake affinity, it was subjected to 90 generations of laboratory evolution in anaerobic, sucrose-limited chemostat cultivation, resulting in a 20-fold decrease of residual sucrose concentrations and a 10-fold increase of the sucrose-transport capacity. A single-cell isolate showed an 11% higher ethanol yield on sucrose in chemostat cultures than an isogenic SUC2 reference strain, while transcriptome analysis revealed elevated expression of AGT1, encoding a disaccharide-proton symporter, and other maltose-related genes. After deletion of both copies of the duplicated AGT1, growth characteristics reverted to that of the unevolved SUC2 and iSUC2 strains. This study demonstrates that engineering the topology of sucrose metabolism is an attractive strategy to improve ethanol yields in industrial processes." @default.
• W2035314369 created "2016-06-24" @default.
• W2035314369 creator A5014224392 @default.
• W2035314369 creator A5028316125 @default.
• W2035314369 creator A5033414409 @default.
• W2035314369 creator A5035562551 @default.
• W2035314369 creator A5050886035 @default.
• W2035314369 creator A5051149804 @default.
• W2035314369 creator A5054970627 @default.
• W2035314369 creator A5061898658 @default.
• W2035314369 creator A5075761769 @default.
• W2035314369 creator A5076553798 @default.
• W2035314369 creator A5087000945 @default.
• W2035314369 creator A5088155093 @default.
• W2035314369 creator A5088420553 @default.
• W2035314369 date "2011-11-01" @default.
• W2035314369 modified "2023-10-16" @default.
• W2035314369 title "Engineering topology and kinetics of sucrose metabolism in Saccharomyces cerevisiae for improved ethanol yield" @default.
• W2035314369 cites W1483456869 @default.
• W2035314369 cites W1506245646 @default.
• W2035314369 cites W1517466915 @default.
• W2035314369 cites W1524703060 @default.
• W2035314369 cites W1757920939 @default.
• W2035314369 cites W1968463000 @default.
• W2035314369 cites W1969859852 @default.
• W2035314369 cites W1973851828 @default.
• W2035314369 cites W1980315658 @default.
• W2035314369 cites W1984096521 @default.
• W2035314369 cites W1985000441 @default.
• W2035314369 cites W1987075204 @default.
• W2035314369 cites W1988884687 @default.
• W2035314369 cites W1989622532 @default.
• W2035314369 cites W1992647465 @default.
• W2035314369 cites W1996231038 @default.
• W2035314369 cites W2001615843 @default.
• W2035314369 cites W2003675373 @default.
• W2035314369 cites W2008141632 @default.
• W2035314369 cites W2008270905 @default.
• W2035314369 cites W2013767271 @default.
• W2035314369 cites W2015265587 @default.
• W2035314369 cites W2017207712 @default.
• W2035314369 cites W2024109444 @default.
• W2035314369 cites W2025239621 @default.
• W2035314369 cites W2033356767 @default.
• W2035314369 cites W2036545186 @default.
• W2035314369 cites W2038979858 @default.
• W2035314369 cites W2047150231 @default.
• W2035314369 cites W2057511197 @default.
• W2035314369 cites W2061279096 @default.
• W2035314369 cites W2085465508 @default.
• W2035314369 cites W2086271348 @default.
• W2035314369 cites W2088933296 @default.
• W2035314369 cites W2092265987 @default.
• W2035314369 cites W2097588241 @default.
• W2035314369 cites W2098839738 @default.
• W2035314369 cites W2099830000 @default.
• W2035314369 cites W2101442503 @default.
• W2035314369 cites W2103017472 @default.
• W2035314369 cites W2115425788 @default.
• W2035314369 cites W2116734717 @default.
• W2035314369 cites W2118625346 @default.
• W2035314369 cites W2120376302 @default.
• W2035314369 cites W2121206586 @default.
• W2035314369 cites W2126359414 @default.
• W2035314369 cites W2128284598 @default.
• W2035314369 cites W2145265114 @default.
• W2035314369 cites W2152232041 @default.
• W2035314369 cites W2156663873 @default.
• W2035314369 cites W2156780012 @default.
• W2035314369 cites W2156847800 @default.
• W2035314369 cites W2159613438 @default.
• W2035314369 cites W2159687706 @default.
• W2035314369 cites W2160530532 @default.
• W2035314369 cites W2162056846 @default.
• W2035314369 cites W2163949203 @default.
• W2035314369 cites W2166866734 @default.
• W2035314369 cites W2167812532 @default.
• W2035314369 cites W2169333620 @default.
• W2035314369 cites W2171435690 @default.
• W2035314369 cites W2171885622 @default.
• W2035314369 cites W2297853161 @default.
• W2035314369 cites W3046397622 @default.
• W2035314369 cites W4294107304 @default.
• W2035314369 cites W70082989 @default.
• W2035314369 doi "https://doi.org/10.1016/j.ymben.2011.09.005" @default.
• W2035314369 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21963484" @default.
• W2035314369 hasPublicationYear "2011" @default.
• W2035314369 type Work @default.
• W2035314369 sameAs 2035314369 @default.
• W2035314369 citedByCount "91" @default.
• W2035314369 countsByYear W20353143692012 @default.
• W2035314369 countsByYear W20353143692013 @default.
• W2035314369 countsByYear W20353143692014 @default.
• W2035314369 countsByYear W20353143692015 @default.
• W2035314369 countsByYear W20353143692016 @default.
• W2035314369 countsByYear W20353143692017 @default.
• W2035314369 countsByYear W20353143692018 @default.
• W2035314369 countsByYear W20353143692019 @default.

• next