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• W2036690749 abstract "The rigorous derivation of the fundamental equation of the dual-mode gradient elution in liquid chromatography involving any type of simultaneous changes in flow rate and mobile-phase composition is developed following Drake's approach. The equation is a generalization of the already known fundamental equations of single gradient when either the mobile-phase composition or the flow rate is constant. The theory was tested in the retention prediction from isocratic data of 18 o-phthalaldehyde derivatives of amino acids in eluting systems modified by acetonitrile or methanol. The retention prediction obtained for all solutes under all dual-mode gradient conditions was excellent. The average percentage error between experimental and predicted retention times ranged from 0.9 to 2.5%. Two approximations that simplify the calculations considerably without increasing the above error were also proposed." @default.
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• W2036690749 date "2007-04-20" @default.
• W2036690749 modified "2023-09-30" @default.
• W2036690749 title "Fundamental Equation of Dual-Mode Gradient Elution in Liquid Chromatography Involving Simultaneous Changes in Flow Rate and Mobile-Phase Composition" @default.
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• W2036690749 doi "https://doi.org/10.1021/ac070090r" @default.
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