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• W2044449035 abstract "Spatiotemporally-controlled delivery of hypoxia-induced angiogenic factor mixtures has been identified by this group as a promising strategy for overcoming the limited ability of chronically ischemic tissues to generate adaptive angiogenesis. We previously developed an implantable, as well as an injectable system for delivering fibroblast-produced factors in vivo. Here, we identify peripheral blood cells (PBCs) as the ideal factor-providing candidates, due to their autologous nature, ease of harvest and ample supply, and investigate wound-simulating biochemical and biophysical environmental parameters that can be controlled to optimize PBC angiogenic activity. It was found that hypoxia (3% O₂) significantly affected the expression of a range of angiogenesis-related factors including VEGF, angiogenin and thrombospondin-1, relative to the normoxic baseline. While all three factors underwent down-regulation over time under hypoxia, there was significant variation in the temporal profile of their expression. VEGF expression was also found to be dependent on cell-scaffold material composition, with fibrin stimulating production the most, followed by collagen and polystyrene. Cell-scaffold matrix stiffness was an additional important factor, as shown by higher VEGF protein levels when PBCs were cultured on stiff vs. compliant collagen hydrogel scaffolds. Engineered PBC-derived factor mixtures could be harvested within cell-free gel and microsphere carriers. The angiogenic effectiveness of factor-loaded carriers could be demonstrated by the ability of their releasates to induce endothelial cell tubule formation and directional migration in in vitro Matrigel assays, and microvessel sprouting in the aortic ring assay. To aid the clinical translation of this approach, we propose a device design that integrates this system, and enables one-step harvesting and delivering of angiogenic factor protein mixtures from autologous peripheral blood. This will facilitate the controlled release of these factors both at the bed-side, as an angiogenic therapy in wounds and peripheral ischemic tissue, as well as pre-, intra- and post-operatively as angiogenic support for central ischemic tissue, grafts, flaps and tissue engineered implants." @default.
• W2044449035 created "2016-06-24" @default.
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• W2044449035 date "2013-07-01" @default.
• W2044449035 modified "2023-10-17" @default.
• W2044449035 title "Cell-free carrier system for localized delivery of peripheral blood cell-derived engineered factor signaling: towards development of a one-step device for autologous angiogenic therapy" @default.
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• W2044449035 cites W1886456063 @default.
• W2044449035 cites W1967944975 @default.
• W2044449035 cites W1970267825 @default.
• W2044449035 cites W1974242642 @default.
• W2044449035 cites W1974796552 @default.
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• W2044449035 cites W2029279493 @default.
• W2044449035 cites W2037753982 @default.
• W2044449035 cites W2043896159 @default.
• W2044449035 cites W2047607520 @default.
• W2044449035 cites W2051010380 @default.
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• W2044449035 cites W2063140011 @default.
• W2044449035 cites W2067081942 @default.
• W2044449035 cites W2073677041 @default.
• W2044449035 cites W2081720885 @default.
• W2044449035 cites W2082605815 @default.
• W2044449035 cites W2087266711 @default.
• W2044449035 cites W2093769042 @default.
• W2044449035 cites W2095170053 @default.
• W2044449035 cites W2097134490 @default.
• W2044449035 cites W2101201145 @default.
• W2044449035 cites W2106324055 @default.
• W2044449035 cites W2111897045 @default.
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• W2044449035 doi "https://doi.org/10.1016/j.jconrel.2013.04.008" @default.
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