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• W2047702904 abstract "The effects of 8-ethoxycaffeine (EOC), adenine, and deoxyadenosine (AdR) on nucleic acid synthesis and the levels of adenosine 5′-triphosphate (ATP) and deoxyadenosine 5′-triphosphate (dATP) in excised and intact bean root tips have been studied. As shown by biochemical and autoradiographic experiments all three purine derivatives produced a strong inhibition of the synthesis of DNA. RNA synthesis was strongly inhibited by EOC and adenine, but only very slightly by AdR. In the biochemical experiments, nucleic acid synthesis was determined by measuring the incorporation of 32 P-labelled phosphate or 14 C-labelled thymidine into the DNA and 32 P-labelled phosphate into the RNA fractions of the roots; in the autoradiographic experiments the incorporation of tritium-labelled thymidine into nuclear DNA was studied. Treatments with AdR resulted in an accumulation of dATP in the root tips. The level of dATP was not affected by adenine, but appeared to be slightly increased by EOC. About a 40% increase of the ATP level was obtained with EOC in the absence of inorganic phosphate. In the presence of a 6.67 mM phosphate buffer, the ATP level was reduced by EOC to one-third of its normal value. Treatment with adenine resulted in a doubling of the ATP level in the roots both in the presence and in the absence of phosphate. The results and their possible significance for the chromosome-breaking effect of the three purine derivatives are discussed." @default.
• W2047702904 created "2016-06-24" @default.
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• W2047702904 date "1965-06-01" @default.
• W2047702904 modified "2023-09-29" @default.
• W2047702904 title "Effects of chromosome-breaking purine derivatives on nucleic acid synthesis and on the levels of adenosine 5′-triphosphate and deoxyadenosine 5′-triphosphate in bean root tips" @default.
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• W2047702904 doi "https://doi.org/10.1016/0027-5107(65)90038-2" @default.
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