FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2058014722> ?p ?o ?g. }

• W2058014722 endingPage "32" @default.
• W2058014722 startingPage "19" @default.
• W2058014722 abstract "2-CL-IB-MECA, (A3 adenosine receptor agonist)(A3AR) mediated cardioprotection is well documented although the associated intracellular signalling pathways remain unclear. Here we demonstrate a role of the pro-survival signalling pathways MEK1/2-ERK1/2 and PI3K/AKT and their effect on modifying Caspase-3 activity in A3AR mediated cardioprotection. Isolated perfused rat hearts or primary adult rat cardiac myocytes were subjected to ischaemia/hypoxia and reperfusion/reoxygenation, respectively. 2-CL-IB-MECA (1 nM) was administered at the onset of reperfusion/reoxygenation in the presence and absence of either the PI3K inhibitor Wortmannin (5 nM) or MEK1/2 inhibitor UO126 (10 μM). Heart tissues were harvested for assessment of p-ERK1/2(Thr202/Tyr204) or p-AKT (Ser-473) status or underwent infarct size assessment. Cardiac myocytes underwent flow-cytometric analysis for apoptosis, necrosis, cleaved-caspase 3/p-BAD (Ser-112 and Ser-136) activity post-reoxygenation. 2-CL-IB-MECA significantly reduced infarct size compared to non-treated controls, where co-administration with either of the kinase inhibitors abolished the infarct sparing effects. Administration of 2-CL-IB-MECA at reperfusion significantly upregulated the status of p-ERK1/2 and p-AKT compared to time matched controls in a UO126 and Wortmannin sensitive manner respectively. 2-CL-IB-MECA when administered throughout reoxygenation significantly reduced apoptosis, necrosis, cleaved-caspase 3 activity and increased p-BAD (Ser-112) and p-BAD (Ser-136) activity in myocytes subjected to hypoxia/reoxygenation injury. The cytoprotective effect was abolished by co-administration with the kinase inhibitors Wortmannin and/or UO126. We have described the molecular mechanisms associated with A3AR mediated cardioprotection indicating a role for the pro-survival signalling pathways that decrease caspase-3 activity. These observations provide novel insight into the pharmacological effects of A3ARs in ameliorating myocardial ischaemia/reperfusion injury." @default.
• W2058014722 created "2016-06-24" @default.
• W2058014722 creator A5031580450 @default.
• W2058014722 creator A5035769758 @default.
• W2058014722 creator A5052811451 @default.
• W2058014722 creator A5075740845 @default.
• W2058014722 date "2013-11-14" @default.
• W2058014722 modified "2023-09-29" @default.
• W2058014722 title "Caspase Inhibition Via A3 Adenosine Receptors: A New Cardioprotective Mechanism Against Myocardial Infarction" @default.
• W2058014722 cites W1658072656 @default.
• W2058014722 cites W1969912131 @default.
• W2058014722 cites W1971724362 @default.
• W2058014722 cites W1974393039 @default.
• W2058014722 cites W1977134430 @default.
• W2058014722 cites W1980336594 @default.
• W2058014722 cites W1986161535 @default.
• W2058014722 cites W1986457074 @default.
• W2058014722 cites W1987664769 @default.
• W2058014722 cites W1990921820 @default.
• W2058014722 cites W2002828754 @default.
• W2058014722 cites W2004700698 @default.
• W2058014722 cites W2005980194 @default.
• W2058014722 cites W2010807686 @default.
• W2058014722 cites W2013281633 @default.
• W2058014722 cites W2028829631 @default.
• W2058014722 cites W2032354327 @default.
• W2058014722 cites W2037404604 @default.
• W2058014722 cites W2044846711 @default.
• W2058014722 cites W2057761726 @default.
• W2058014722 cites W2059275861 @default.
• W2058014722 cites W2066095390 @default.
• W2058014722 cites W2067846325 @default.
• W2058014722 cites W2069697226 @default.
• W2058014722 cites W2075206212 @default.
• W2058014722 cites W2077259639 @default.
• W2058014722 cites W2079387700 @default.
• W2058014722 cites W2080784615 @default.
• W2058014722 cites W2083010337 @default.
• W2058014722 cites W2087881873 @default.
• W2058014722 cites W2088746083 @default.
• W2058014722 cites W2090052077 @default.
• W2058014722 cites W2094064134 @default.
• W2058014722 cites W2110412870 @default.
• W2058014722 cites W2111604665 @default.
• W2058014722 cites W2116899872 @default.
• W2058014722 cites W2122057254 @default.
• W2058014722 cites W2126214253 @default.
• W2058014722 cites W2127181417 @default.
• W2058014722 cites W2131621174 @default.
• W2058014722 cites W2137360409 @default.
• W2058014722 cites W2144317957 @default.
• W2058014722 cites W2147791296 @default.
• W2058014722 cites W2155668811 @default.
• W2058014722 cites W2161686109 @default.
• W2058014722 cites W2163982472 @default.
• W2058014722 cites W2167846804 @default.
• W2058014722 cites W2327972260 @default.
• W2058014722 cites W2417798102 @default.
• W2058014722 doi "https://doi.org/10.1007/s10557-013-6500-y" @default.
• W2058014722 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24220799" @default.
• W2058014722 hasPublicationYear "2013" @default.
• W2058014722 type Work @default.
• W2058014722 sameAs 2058014722 @default.
• W2058014722 citedByCount "30" @default.
• W2058014722 countsByYear W20580147222014 @default.
• W2058014722 countsByYear W20580147222015 @default.
• W2058014722 countsByYear W20580147222016 @default.
• W2058014722 countsByYear W20580147222017 @default.
• W2058014722 countsByYear W20580147222018 @default.
• W2058014722 countsByYear W20580147222019 @default.
• W2058014722 countsByYear W20580147222021 @default.
• W2058014722 countsByYear W20580147222022 @default.
• W2058014722 countsByYear W20580147222023 @default.
• W2058014722 crossrefType "journal-article" @default.
• W2058014722 hasAuthorship W2058014722A5031580450 @default.
• W2058014722 hasAuthorship W2058014722A5035769758 @default.
• W2058014722 hasAuthorship W2058014722A5052811451 @default.
• W2058014722 hasAuthorship W2058014722A5075740845 @default.
• W2058014722 hasBestOaLocation W20580147222 @default.
• W2058014722 hasConcept C126322002 @default.
• W2058014722 hasConcept C134018914 @default.
• W2058014722 hasConcept C178790620 @default.
• W2058014722 hasConcept C185592680 @default.
• W2058014722 hasConcept C190283241 @default.
• W2058014722 hasConcept C207200792 @default.
• W2058014722 hasConcept C2776991684 @default.
• W2058014722 hasConcept C2778308172 @default.
• W2058014722 hasConcept C2779676291 @default.
• W2058014722 hasConcept C2780114680 @default.
• W2058014722 hasConcept C540031477 @default.
• W2058014722 hasConcept C541997718 @default.
• W2058014722 hasConcept C55493867 @default.
• W2058014722 hasConcept C71924100 @default.
• W2058014722 hasConcept C75217442 @default.
• W2058014722 hasConcept C7836513 @default.
• W2058014722 hasConcept C86554907 @default.
• W2058014722 hasConcept C86803240 @default.
• W2058014722 hasConcept C98274493 @default.

• next