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• W2058353670 abstract "In the presence of Cl−, the severity of ammonia-induced inhibition of photosynthetic oxygen evolution is attenuated in spinach thylakoid membranes (Sandusky, P.O. and Yocum, C.F. (1983) FEBS Lett. 162, 339–343). A further examination of this phenomenon using steady-state kinetic analysis suggests that there are two sites of ammonia attack, only one of which is protected by the presence of Cl−. In the case of Tris-induced inhibition of oxygen evolution only the Cl− protected site is evident. In both cases the mechanism of Cl− protection involves the binding of Cl− in competition with the inhibitory amine. Anions (Br− and NO−3) known to reactive oxygen evolution in Cl−-depleted membranes also protect against Tris-induced inhibition, and reactivation of Cl−-depleted membranes by Cl− is competitively inhibited by ammonia. Inactivation of the oxygen-evolving complex by NH2OH is impeded by Cl−, whereas Cl− does not affect the inhibition induced by so-called ADRY reagents. We propose that Cl− functions in the oxygen-evolving complex as a ligand bridging manganese atoms to mediate electron transfer. This model accounts both for the well known Cl− requirement of oxygen evolution, and for the inhibitory effects of amines on this reaction." @default.
• W2058353670 created "2016-06-24" @default.
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• W2058353670 date "1984-09-01" @default.
• W2058353670 modified "2023-09-26" @default.
• W2058353670 title "The chloride requirement for photosynthetic oxygen evolution. Analysis of the effects of chloride and other anions on amine inhibition of the oxygen-evolving complex" @default.
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• W2058353670 doi "https://doi.org/10.1016/0005-2728(84)90121-x" @default.
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