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- W2059804911 abstract "Using genetic engineering techniques we generated randomly located internal tandem duplications of random size within Staphylococcal nuclease. Those insertions, possessing greater than 0.1% of normal activity, were sequenced and characterized physically. Insertions were found to begin and end in regions possessing secondary structure as well as in regions without secondary structure. All proteins remained folded and monomeric, although one mutant appeared, by both circular dichroism and size exclusion chromatography, to be partially unfolded. The stability of the insertions as assayed by guanidine hydrochloride denaturation ranged from nearly normal to destabilized by almost 4 kcal per mol. The activities of the insertion mutants ranged from 1/30 to 1/2000 of the parental nuclease." @default.
- W2059804911 created "2016-06-24" @default.
- W2059804911 creator A5071638721 @default.
- W2059804911 creator A5077349136 @default.
- W2059804911 date "1998-10-01" @default.
- W2059804911 modified "2023-09-28" @default.
- W2059804911 title "Isolation and physical characterization of random insertions in staphylococcal nuclease" @default.
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- W2059804911 doi "https://doi.org/10.1006/jmbi.1998.2063" @default.
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