Matches in SemOpenAlex for { <https://semopenalex.org/work/W2069369854> ?p ?o ?g. }
- W2069369854 endingPage "2337" @default.
- W2069369854 startingPage "2330" @default.
- W2069369854 abstract "The E6 oncoprotein of high-risk human papillomavirus type 16 (HPV16) interacts with several nuclear transcription factors and coactivators in addition to cytoplasmic proteins, suggesting that nuclear import of HPV16 E6 plays a role in the cellular transformation process. In this study we have investigated the nuclear import pathways of HPV16 E6 in digitonin-permeabilized HeLa cells. We found that HPV16 E6 interacted with the karyopherin (Kap) alpha2 adapter and could enter the nucleus via a classical Kap alpha2beta1-mediated pathway. Interestingly, HPV16 E6 also interacted, via its basic nuclear localization signal (NLS) located at the C terminus, with both Kap beta1 and Kap beta2 import receptors. Binding of RanGTP to these Kap betas inhibited their interaction with HPV16 E6 NLS. In agreement with these binding data, nuclear import of the HPV16 E6 oncoprotein in digitonin-permeabilized HeLa cells could be mediated by either Kap beta1 or Kap beta2. Nuclear import via these pathways required RanGDP and was independent of GTP hydrolysis by Ran. Significantly, an E6(R124G) mutant had reduced nuclear import activity, and the E6 deletion mutant lacking (121)KKQR(124) was not imported into the nucleus. The data reveal that the HPV16 E6 oncoprotein interacts via its C-terminal NLS with several karyopherins and exploits these interactions to enter the nucleus of host cells via multiple pathways." @default.
- W2069369854 created "2016-06-24" @default.
- W2069369854 creator A5019300808 @default.
- W2069369854 creator A5091871114 @default.
- W2069369854 date "2003-02-15" @default.
- W2069369854 modified "2023-09-26" @default.
- W2069369854 title "Nuclear Entry of High-Risk Human Papillomavirus Type 16 E6 Oncoprotein Occurs via Several Pathways" @default.
- W2069369854 cites W140061535 @default.
- W2069369854 cites W1493793804 @default.
- W2069369854 cites W1559244741 @default.
- W2069369854 cites W1605063081 @default.
- W2069369854 cites W1675243257 @default.
- W2069369854 cites W1777838984 @default.
- W2069369854 cites W1856360008 @default.
- W2069369854 cites W1965806742 @default.
- W2069369854 cites W1969081197 @default.
- W2069369854 cites W1971660947 @default.
- W2069369854 cites W1972518578 @default.
- W2069369854 cites W1973731625 @default.
- W2069369854 cites W1979906352 @default.
- W2069369854 cites W1982770994 @default.
- W2069369854 cites W1985752008 @default.
- W2069369854 cites W1986169988 @default.
- W2069369854 cites W1992795861 @default.
- W2069369854 cites W1998849903 @default.
- W2069369854 cites W2000110812 @default.
- W2069369854 cites W2001788009 @default.
- W2069369854 cites W2002017977 @default.
- W2069369854 cites W2008415422 @default.
- W2069369854 cites W2008984155 @default.
- W2069369854 cites W2014047301 @default.
- W2069369854 cites W2016735382 @default.
- W2069369854 cites W2017284082 @default.
- W2069369854 cites W2022606160 @default.
- W2069369854 cites W2026008221 @default.
- W2069369854 cites W2033951100 @default.
- W2069369854 cites W2039382131 @default.
- W2069369854 cites W2040029052 @default.
- W2069369854 cites W2051948296 @default.
- W2069369854 cites W2054813501 @default.
- W2069369854 cites W2073409278 @default.
- W2069369854 cites W2075312092 @default.
- W2069369854 cites W2078356604 @default.
- W2069369854 cites W2092515268 @default.
- W2069369854 cites W2094134169 @default.
- W2069369854 cites W2095460693 @default.
- W2069369854 cites W2103755904 @default.
- W2069369854 cites W2108720228 @default.
- W2069369854 cites W2112534911 @default.
- W2069369854 cites W2119064030 @default.
- W2069369854 cites W2132672808 @default.
- W2069369854 cites W2138688363 @default.
- W2069369854 cites W2139573924 @default.
- W2069369854 cites W2140014254 @default.
- W2069369854 cites W2143019738 @default.
- W2069369854 cites W2146018121 @default.
- W2069369854 cites W2146581496 @default.
- W2069369854 cites W2147065993 @default.
- W2069369854 cites W2150525034 @default.
- W2069369854 cites W2153258255 @default.
- W2069369854 cites W2165306451 @default.
- W2069369854 cites W2167415438 @default.
- W2069369854 cites W2167715597 @default.
- W2069369854 cites W2170939558 @default.
- W2069369854 cites W2171185708 @default.
- W2069369854 cites W2188111130 @default.
- W2069369854 cites W2804367574 @default.
- W2069369854 cites W4230507395 @default.
- W2069369854 cites W4248964848 @default.
- W2069369854 cites W4250214354 @default.
- W2069369854 cites W4366064175 @default.
- W2069369854 doi "https://doi.org/10.1128/jvi.77.4.2330-2337.2003" @default.
- W2069369854 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/141087" @default.
- W2069369854 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12551970" @default.
- W2069369854 hasPublicationYear "2003" @default.
- W2069369854 type Work @default.
- W2069369854 sameAs 2069369854 @default.
- W2069369854 citedByCount "42" @default.
- W2069369854 countsByYear W20693698542012 @default.
- W2069369854 countsByYear W20693698542013 @default.
- W2069369854 countsByYear W20693698542014 @default.
- W2069369854 countsByYear W20693698542016 @default.
- W2069369854 countsByYear W20693698542017 @default.
- W2069369854 countsByYear W20693698542019 @default.
- W2069369854 countsByYear W20693698542020 @default.
- W2069369854 countsByYear W20693698542021 @default.
- W2069369854 countsByYear W20693698542023 @default.
- W2069369854 crossrefType "journal-article" @default.
- W2069369854 hasAuthorship W2069369854A5019300808 @default.
- W2069369854 hasAuthorship W2069369854A5091871114 @default.
- W2069369854 hasBestOaLocation W20693698542 @default.
- W2069369854 hasConcept C104317684 @default.
- W2069369854 hasConcept C126725582 @default.
- W2069369854 hasConcept C133882971 @default.
- W2069369854 hasConcept C153911025 @default.
- W2069369854 hasConcept C160704184 @default.
- W2069369854 hasConcept C181199279 @default.
- W2069369854 hasConcept C186435350 @default.