FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2077804546> ?p ?o ?g. }

• W2077804546 endingPage "10604" @default.
• W2077804546 startingPage "10596" @default.
• W2077804546 abstract "The Herpes Simplex Virion Protein 16 (VP16) activates transcription through a series of protein/protein interactions involving its highly acidic transactivation domain (TAD). The acidic TAD of VP16 (VP16TAD) has been shown to interact with several partner proteins both in vitro and in vivo, and many of these VP16 partners also bind the acidic TAD of the mammalian tumor suppressor protein p53. For example, the TADs of VP16 and p53 (p53TAD) both interact directly with the p62/Tfb1 (human/yeast) subunit of TFIIH, and this interaction correlates with their ability to activate both the initiation and elongation phase of transcription. In this manuscript, we use NMR spectroscopy, isothermal titration calorimetery (ITC) and site-directed mutagenesis studies to characterize the interaction between the VP16TAD and Tfb1. We identify a region within the carboxyl-terminal subdomain of the VP16TAD (VP16C) that has sequence similarity with p53TAD2 and binds Tfb1 with nanomolar affinity. We determine an NMR structure of a Tfb1/VP16C complex, which represents the first high-resolution structure of the VP16TAD in complex with a target protein. The structure demonstrates that like p53TAD2, VP16C forms a 9-residue α-helix in complex with Tfb1. Comparison of the VP16/Tfb1and p53/Tfb1 structures clearly demonstrates how the viral activator VP16C and p53TAD2 shares numerous aspects of binding to Tfb1. Despite the similarities, important differences are observed between the p53TAD2/Tfb1 and VP16C/Tfb1 complexes, and these differences demonstrate how selected activators such as p53 depend on phosphorylation events to selectively regulate transcription." @default.
• W2077804546 created "2016-06-24" @default.
• W2077804546 creator A5004109558 @default.
• W2077804546 creator A5017046869 @default.
• W2077804546 creator A5024887234 @default.
• W2077804546 creator A5051955080 @default.
• W2077804546 creator A5070289824 @default.
• W2077804546 creator A5089853419 @default.
• W2077804546 date "2008-07-17" @default.
• W2077804546 modified "2023-10-04" @default.
• W2077804546 title "NMR Structure of the Complex between the Tfb1 Subunit of TFIIH and the Activation Domain of VP16: Structural Similarities between VP16 and p53" @default.
• W2077804546 cites W1494657683 @default.
• W2077804546 cites W1507254050 @default.
• W2077804546 cites W1589295940 @default.
• W2077804546 cites W1615402573 @default.
• W2077804546 cites W1907444180 @default.
• W2077804546 cites W1970444492 @default.
• W2077804546 cites W1975459052 @default.
• W2077804546 cites W1976261939 @default.
• W2077804546 cites W1976873804 @default.
• W2077804546 cites W1982756681 @default.
• W2077804546 cites W1983825024 @default.
• W2077804546 cites W1988565419 @default.
• W2077804546 cites W1989745913 @default.
• W2077804546 cites W1990854276 @default.
• W2077804546 cites W1991494214 @default.
• W2077804546 cites W1998063596 @default.
• W2077804546 cites W2000027744 @default.
• W2077804546 cites W2000388754 @default.
• W2077804546 cites W2000678537 @default.
• W2077804546 cites W2002195659 @default.
• W2077804546 cites W2006323829 @default.
• W2077804546 cites W2008167615 @default.
• W2077804546 cites W2008693523 @default.
• W2077804546 cites W2015492400 @default.
• W2077804546 cites W2015606278 @default.
• W2077804546 cites W2016888788 @default.
• W2077804546 cites W2019831987 @default.
• W2077804546 cites W2020484813 @default.
• W2077804546 cites W2021825316 @default.
• W2077804546 cites W2027216514 @default.
• W2077804546 cites W2030816023 @default.
• W2077804546 cites W2037579319 @default.
• W2077804546 cites W2038630278 @default.
• W2077804546 cites W2041807810 @default.
• W2077804546 cites W2042688292 @default.
• W2077804546 cites W2046117901 @default.
• W2077804546 cites W2046121745 @default.
• W2077804546 cites W2048880554 @default.
• W2077804546 cites W2051210276 @default.
• W2077804546 cites W2052529174 @default.
• W2077804546 cites W2052606462 @default.
• W2077804546 cites W2053700118 @default.
• W2077804546 cites W2054525948 @default.
• W2077804546 cites W2058402081 @default.
• W2077804546 cites W2059185804 @default.
• W2077804546 cites W2068577315 @default.
• W2077804546 cites W2071125128 @default.
• W2077804546 cites W2071262787 @default.
• W2077804546 cites W2072364431 @default.
• W2077804546 cites W2076191017 @default.
• W2077804546 cites W2080796053 @default.
• W2077804546 cites W2080918768 @default.
• W2077804546 cites W2082176639 @default.
• W2077804546 cites W2087950896 @default.
• W2077804546 cites W2091160336 @default.
• W2077804546 cites W2091423718 @default.
• W2077804546 cites W2091631845 @default.
• W2077804546 cites W2095491514 @default.
• W2077804546 cites W2102025585 @default.
• W2077804546 cites W2104353983 @default.
• W2077804546 cites W2105014300 @default.
• W2077804546 cites W2114151755 @default.
• W2077804546 cites W2116239679 @default.
• W2077804546 cites W2119383396 @default.
• W2077804546 cites W2120552994 @default.
• W2077804546 cites W2122794297 @default.
• W2077804546 cites W2124129756 @default.
• W2077804546 cites W2126521510 @default.
• W2077804546 cites W2126568240 @default.
• W2077804546 cites W2130289709 @default.
• W2077804546 cites W2139146047 @default.
• W2077804546 cites W2146615455 @default.
• W2077804546 cites W2149168187 @default.
• W2077804546 cites W2165762583 @default.
• W2077804546 cites W2169821755 @default.
• W2077804546 cites W2334062824 @default.
• W2077804546 cites W2334464812 @default.
• W2077804546 doi "https://doi.org/10.1021/ja800975h" @default.
• W2077804546 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18630911" @default.
• W2077804546 hasPublicationYear "2008" @default.
• W2077804546 type Work @default.
• W2077804546 sameAs 2077804546 @default.
• W2077804546 citedByCount "52" @default.
• W2077804546 countsByYear W20778045462012 @default.
• W2077804546 countsByYear W20778045462013 @default.
• W2077804546 countsByYear W20778045462014 @default.
• W2077804546 countsByYear W20778045462015 @default.

• next