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- W2078889788 abstract "The performance of Magarose®, an agarose-based bead containing a paramagnetic component has been evaluated. The anion exchanger DEAE-Magarose is effective at binding DNA from a crude cell lysate. The plasmid pBluescript was isolated from 1.5 ml Escherichia coli JM109 cell culture, following alkaline lysis yielding 8.2 μg high-quality DNA. Under similar binding conditions 21 μg of salmon sperm DNA bound to the ion exchangers. The affinity medium oligo-dT Magarose was demonstrated to bind 75 μmol of an oligo-dA probe/g of medium by hybridization. Under similar conditions mRNA could be isolated from a preparation of baby hamster cell total RNA. The magnetic susceptibility of Magarose is very high, facilitating the use of this separation technique for rapid batch chromatographic processes." @default.
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- W2078889788 date "1998-08-01" @default.
- W2078889788 modified "2023-09-27" @default.
- W2078889788 title "Recent developments of magnetic beads for use in nucleic acid purification" @default.
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- W2078889788 doi "https://doi.org/10.1016/s0021-9673(98)00064-8" @default.
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