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• W2081085370 abstract "The activities of 5 key regulatory enzymes in most energetic systems, namely citrate synthase (EC 4.1.3.7, CS), NADP-specific isocitrate dehydrogenase (EC 1.1.1.42, ICDH), succinate dehydrogenase (EC 1.3.99.1, SDH), L-malate dehydrogenase (EC 1.1.1.37, MDH), and decarboxylating malic enzyme (EC 1.1.1.40, ME), were measured during the growth and metacyclogenesis of a cutaneous (CL) and a visceral (VL) strain of Leishmania infantum. As occurs with other Leishmania species, infective promastigotes were present along all phases of growth, but their percentages were higher at the early stationary phase for VL and the end of the same phase for CL. High CS and SDH activities were detected in both strains, as compared with other trypanosomatids, bringing more evidence for an actively functional citric-acid cycle in L. infantum. Both strains showed higher levels of CS, ICDH, and MDH and lower SDH and ME activities when more metacyclic promastigotes were present, but in VL these changes paralleled an increase in glucose consumption, whereas in CL these changes coincided with an NH3 hyperproduction. This suggests that the energy metabolism during L. infantum growth and metacyclogenesis is affected by regulated enzymes that probably respond to changes in the culture medium in the levels of glucose and amino acids." @default.
• W2081085370 created "2016-06-24" @default.
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• W2081085370 date "1999-08-01" @default.
• W2081085370 modified "2023-09-27" @default.
• W2081085370 title "Citric-Acid Cycle Key Enzyme Activities during In vitro Growth and Metacyclogenesis of Leishmania infantum Promastigotes" @default.
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• W2081085370 doi "https://doi.org/10.2307/3285729" @default.
• W2081085370 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10461937" @default.
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