FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2083531486> ?p ?o ?g. }

• W2083531486 endingPage "1702" @default.
• W2083531486 startingPage "1696" @default.
• W2083531486 abstract "We have established an assay based on gas chromatography-mass spectrometry to profile and quantitate endogenous cytochrome P450 monooxygenase (P450)-hydroxyeicosatetraenoic acids (HETEs) exiting the isolated perfused rabbit kidney in response to hormonal stimulation. In response to angiotensin II (Ang II) P450-derived HETEs (16-, 17-, 18-, 19- and 20-) are released from the isolated Kreb's perfused rabbit kidney. Ang II produced a several-fold increase in the levels of P450-HETEs above basal levels in both urinary (such as for 20-HETE from 0.93 +/- 0.7 to 2.31 +/- 0.9 ng/min) and venous (from 0.1 +/- 0.05 to 0.3 +/- 0.05 ng/min) effluents. However, inhibition of P450, which reduced basal release, did not prevent Ang II-induced release of P450-AA products from the rabbit kidney; for example, urinary 20-HETE in the presence of 17-ODYA (1 microM) was undetectable and increased to 0.93 +/- 0.4 ng/min with Ang II and venous 20-HETE increased from 0.06 +/- 0.03 to 0.24 +/- 0.07 ng/min. Similar results were obtained with clotrimazole (1 microM). As 16-, 18-, 19- and 20-HETEs are vasodilators in the rabbit kidney and 16- and 17-HETEs inhibit proximal tubular ATPase activity, we investigated their possible sites of esterification. Cortical and medullary lipids were extracted, separated by HPLC and P450-HETEs quantitated following alkaline hydrolysis. The P450-HETEs were incorporated into both neutral lipids (NL) and phospholipids [phosphatidylethanolamine (PE), phosphatidylinositol (PI), phosphatidylserine (PS) and phosphatidylcholine (PC)]. However, the assignment of a HETE to a specific phospholipid pool must be regarded as tentative as the appropriate standards containing P450-HETEs in the Sn-2 position (such as 20-HETE-PF., 20-HETE-PC, etc.) were not available. Esterified HETEs were found in larger quantities in the cortex as compared to the medulla (34.40 +/- 1.12 versus 22.76 +/- 0.53 ng/g). The PI fraction in the cortex yielded the largest quantity of HETEs and the PC fraction the lowest. In the medulla, the largest quantities of esterified HETEs were found in neutral lipids and only slightly lesser amounts in PE and PI. Esterified 18-HETE was localized only to the NI fraction. This fraction also contained the other HETEs, 19- and 20-HETE being the most abundant. Notably only 16- and 17-HETE were present in PF, whereas, 19- and 20-HETE were also present in PI, PS and PC. Thus, P450-HETEs, like EETs are stored in the kidney and are, presumably, subject to release by peptide activation of acylhydrolases." @default.
• W2083531486 created "2016-06-24" @default.
• W2083531486 creator A5021612275 @default.
• W2083531486 creator A5045860181 @default.
• W2083531486 creator A5050941164 @default.
• W2083531486 creator A5069103014 @default.
• W2083531486 creator A5077043354 @default.
• W2083531486 creator A5077198825 @default.
• W2083531486 date "1997-06-01" @default.
• W2083531486 modified "2023-10-16" @default.
• W2083531486 title "Cytochrome P450-derived renal HETEs: Storage and release" @default.
• W2083531486 cites W1502959976 @default.
• W2083531486 cites W1513897103 @default.
• W2083531486 cites W1527849891 @default.
• W2083531486 cites W1529262696 @default.
• W2083531486 cites W1549591697 @default.
• W2083531486 cites W1578135150 @default.
• W2083531486 cites W1983062366 @default.
• W2083531486 cites W1998229441 @default.
• W2083531486 cites W2018198144 @default.
• W2083531486 cites W2026414674 @default.
• W2083531486 cites W2041369194 @default.
• W2083531486 cites W2077963565 @default.
• W2083531486 cites W2080289348 @default.
• W2083531486 cites W2131861405 @default.
• W2083531486 cites W2152311269 @default.
• W2083531486 cites W2172746001 @default.
• W2083531486 cites W2185536468 @default.
• W2083531486 cites W2346713216 @default.
• W2083531486 cites W2349327624 @default.
• W2083531486 cites W2394661960 @default.
• W2083531486 cites W2398643093 @default.
• W2083531486 doi "https://doi.org/10.1038/ki.1997.234" @default.
• W2083531486 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9186856" @default.
• W2083531486 hasPublicationYear "1997" @default.
• W2083531486 type Work @default.
• W2083531486 sameAs 2083531486 @default.
• W2083531486 citedByCount "100" @default.
• W2083531486 countsByYear W20835314862012 @default.
• W2083531486 countsByYear W20835314862013 @default.
• W2083531486 countsByYear W20835314862014 @default.
• W2083531486 countsByYear W20835314862015 @default.
• W2083531486 countsByYear W20835314862016 @default.
• W2083531486 countsByYear W20835314862017 @default.
• W2083531486 countsByYear W20835314862018 @default.
• W2083531486 countsByYear W20835314862019 @default.
• W2083531486 countsByYear W20835314862020 @default.
• W2083531486 countsByYear W20835314862021 @default.
• W2083531486 countsByYear W20835314862022 @default.
• W2083531486 crossrefType "journal-article" @default.
• W2083531486 hasAuthorship W2083531486A5021612275 @default.
• W2083531486 hasAuthorship W2083531486A5045860181 @default.
• W2083531486 hasAuthorship W2083531486A5050941164 @default.
• W2083531486 hasAuthorship W2083531486A5069103014 @default.
• W2083531486 hasAuthorship W2083531486A5077043354 @default.
• W2083531486 hasAuthorship W2083531486A5077198825 @default.
• W2083531486 hasBestOaLocation W20835314861 @default.
• W2083531486 hasConcept C126322002 @default.
• W2083531486 hasConcept C13002179 @default.
• W2083531486 hasConcept C134018914 @default.
• W2083531486 hasConcept C181199279 @default.
• W2083531486 hasConcept C184235292 @default.
• W2083531486 hasConcept C185592680 @default.
• W2083531486 hasConcept C2776330855 @default.
• W2083531486 hasConcept C2777477808 @default.
• W2083531486 hasConcept C2778078955 @default.
• W2083531486 hasConcept C2778918659 @default.
• W2083531486 hasConcept C2779630222 @default.
• W2083531486 hasConcept C2780091579 @default.
• W2083531486 hasConcept C2780610907 @default.
• W2083531486 hasConcept C41625074 @default.
• W2083531486 hasConcept C526171541 @default.
• W2083531486 hasConcept C55493867 @default.
• W2083531486 hasConcept C62231903 @default.
• W2083531486 hasConcept C71924100 @default.
• W2083531486 hasConcept C86803240 @default.
• W2083531486 hasConceptScore W2083531486C126322002 @default.
• W2083531486 hasConceptScore W2083531486C13002179 @default.
• W2083531486 hasConceptScore W2083531486C134018914 @default.
• W2083531486 hasConceptScore W2083531486C181199279 @default.
• W2083531486 hasConceptScore W2083531486C184235292 @default.
• W2083531486 hasConceptScore W2083531486C185592680 @default.
• W2083531486 hasConceptScore W2083531486C2776330855 @default.
• W2083531486 hasConceptScore W2083531486C2777477808 @default.
• W2083531486 hasConceptScore W2083531486C2778078955 @default.
• W2083531486 hasConceptScore W2083531486C2778918659 @default.
• W2083531486 hasConceptScore W2083531486C2779630222 @default.
• W2083531486 hasConceptScore W2083531486C2780091579 @default.
• W2083531486 hasConceptScore W2083531486C2780610907 @default.
• W2083531486 hasConceptScore W2083531486C41625074 @default.
• W2083531486 hasConceptScore W2083531486C526171541 @default.
• W2083531486 hasConceptScore W2083531486C55493867 @default.
• W2083531486 hasConceptScore W2083531486C62231903 @default.
• W2083531486 hasConceptScore W2083531486C71924100 @default.
• W2083531486 hasConceptScore W2083531486C86803240 @default.
• W2083531486 hasIssue "6" @default.
• W2083531486 hasLocation W20835314861 @default.
• W2083531486 hasLocation W20835314862 @default.

• next