Matches in SemOpenAlex for { <https://semopenalex.org/work/W2084702912> ?p ?o ?g. }
- W2084702912 endingPage "881" @default.
- W2084702912 startingPage "862" @default.
- W2084702912 abstract "Abstract Proteomics is undergoing a rapid transformation from a qualitative global peptide sequencing discipline into a quantitative, reproducibility‐driven practice. Nowhere is this more evident than in the rapidly expanding field of protein biomarker discovery where the general goal is to uncover statistically robust patterns of differential expression between or among subjects/samples representing distinct biological/temporal states. This report presents the analytical characterization of a label‐free LC FT‐ICR‐MS workflow for differential proteomics analysis of human plasma. The key elements discussed include (i) methodologies for performing properly replicated experiments with highly reproducible sample preparation and analysis, including the use of internal standards to quantify variance at different steps in the process, (ii) a new methodology for performing sample re‐analysis that uses off‐line targeted robotic acquisition of complementary spectral data ( e.g. ECD and/or IRMPD) to enhance the identification of differentially expressed peptides/proteins, and (iii) data processing pipelines capable of integrating the automatic statistical analysis of the label‐free (LC‐) MS signal, together with the intuitive and highly interactive curation and annotation of differential features using the output from standard sequence database search programs. We illustrate the application of the complete sample‐to‐annotated‐differential‐peptides (‐proteins) workflow by describing the acquisition and analysis of a large multidimensional dataset from patients undergoing a controlled myocardial infarction resulting in an experimental setup in which each patients serve as their own control. Furthermore, we discuss a couple illustrative examples of mid‐level proteins observed in this study whose plasma concentrations change consistently within and across patients, in a treatment‐ and time‐dependent fashion." @default.
- W2084702912 created "2016-06-24" @default.
- W2084702912 creator A5005948071 @default.
- W2084702912 creator A5016680524 @default.
- W2084702912 creator A5023928128 @default.
- W2084702912 creator A5039028457 @default.
- W2084702912 creator A5045120054 @default.
- W2084702912 creator A5049804837 @default.
- W2084702912 creator A5049939460 @default.
- W2084702912 date "2008-06-01" @default.
- W2084702912 modified "2023-10-10" @default.
- W2084702912 title "Performance characteristics of an FT MS-based workflow for label-free differential MS analysis of human plasma: standards, reproducibility, targeted feature investigation, and application to a model of controlled myocardial infarction" @default.
- W2084702912 cites W1547169091 @default.
- W2084702912 cites W1575982426 @default.
- W2084702912 cites W1987851205 @default.
- W2084702912 cites W1992271070 @default.
- W2084702912 cites W2011342582 @default.
- W2084702912 cites W2024288744 @default.
- W2084702912 cites W2026465178 @default.
- W2084702912 cites W2033204893 @default.
- W2084702912 cites W2054747743 @default.
- W2084702912 cites W2059560506 @default.
- W2084702912 cites W2065636587 @default.
- W2084702912 cites W2069862631 @default.
- W2084702912 cites W2072671787 @default.
- W2084702912 cites W2073292063 @default.
- W2084702912 cites W2099925531 @default.
- W2084702912 cites W2109156884 @default.
- W2084702912 cites W2110174460 @default.
- W2084702912 cites W2111208057 @default.
- W2084702912 cites W2112078820 @default.
- W2084702912 cites W2120016245 @default.
- W2084702912 cites W2121863513 @default.
- W2084702912 cites W2134749398 @default.
- W2084702912 cites W2140946052 @default.
- W2084702912 cites W2149517572 @default.
- W2084702912 cites W2155792164 @default.
- W2084702912 doi "https://doi.org/10.1002/prca.200780057" @default.
- W2084702912 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21136885" @default.
- W2084702912 hasPublicationYear "2008" @default.
- W2084702912 type Work @default.
- W2084702912 sameAs 2084702912 @default.
- W2084702912 citedByCount "16" @default.
- W2084702912 countsByYear W20847029122012 @default.
- W2084702912 countsByYear W20847029122013 @default.
- W2084702912 countsByYear W20847029122014 @default.
- W2084702912 crossrefType "journal-article" @default.
- W2084702912 hasAuthorship W2084702912A5005948071 @default.
- W2084702912 hasAuthorship W2084702912A5016680524 @default.
- W2084702912 hasAuthorship W2084702912A5023928128 @default.
- W2084702912 hasAuthorship W2084702912A5039028457 @default.
- W2084702912 hasAuthorship W2084702912A5045120054 @default.
- W2084702912 hasAuthorship W2084702912A5049804837 @default.
- W2084702912 hasAuthorship W2084702912A5049939460 @default.
- W2084702912 hasConcept C104317684 @default.
- W2084702912 hasConcept C124101348 @default.
- W2084702912 hasConcept C124535831 @default.
- W2084702912 hasConcept C177212765 @default.
- W2084702912 hasConcept C185592680 @default.
- W2084702912 hasConcept C198531522 @default.
- W2084702912 hasConcept C41008148 @default.
- W2084702912 hasConcept C43617362 @default.
- W2084702912 hasConcept C46111723 @default.
- W2084702912 hasConcept C55493867 @default.
- W2084702912 hasConcept C60644358 @default.
- W2084702912 hasConcept C70721500 @default.
- W2084702912 hasConcept C77088390 @default.
- W2084702912 hasConcept C86803240 @default.
- W2084702912 hasConcept C9893847 @default.
- W2084702912 hasConceptScore W2084702912C104317684 @default.
- W2084702912 hasConceptScore W2084702912C124101348 @default.
- W2084702912 hasConceptScore W2084702912C124535831 @default.
- W2084702912 hasConceptScore W2084702912C177212765 @default.
- W2084702912 hasConceptScore W2084702912C185592680 @default.
- W2084702912 hasConceptScore W2084702912C198531522 @default.
- W2084702912 hasConceptScore W2084702912C41008148 @default.
- W2084702912 hasConceptScore W2084702912C43617362 @default.
- W2084702912 hasConceptScore W2084702912C46111723 @default.
- W2084702912 hasConceptScore W2084702912C55493867 @default.
- W2084702912 hasConceptScore W2084702912C60644358 @default.
- W2084702912 hasConceptScore W2084702912C70721500 @default.
- W2084702912 hasConceptScore W2084702912C77088390 @default.
- W2084702912 hasConceptScore W2084702912C86803240 @default.
- W2084702912 hasConceptScore W2084702912C9893847 @default.
- W2084702912 hasIssue "6" @default.
- W2084702912 hasLocation W20847029121 @default.
- W2084702912 hasLocation W20847029122 @default.
- W2084702912 hasOpenAccess W2084702912 @default.
- W2084702912 hasPrimaryLocation W20847029121 @default.
- W2084702912 hasRelatedWork W1969295409 @default.
- W2084702912 hasRelatedWork W1987164253 @default.
- W2084702912 hasRelatedWork W1999883774 @default.
- W2084702912 hasRelatedWork W2004289232 @default.
- W2084702912 hasRelatedWork W2010715465 @default.
- W2084702912 hasRelatedWork W2031369865 @default.
- W2084702912 hasRelatedWork W2039928317 @default.
- W2084702912 hasRelatedWork W3089769884 @default.
- W2084702912 hasRelatedWork W3150792906 @default.