FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2086587029> ?p ?o ?g. }

• W2086587029 abstract "Protein-protein interactions are pivotal to most, if not all, physiological processes, and understanding the nature of such interactions is a central step in biological research. Surface Plasmon Resonance (SPR) is a sensitive detection technique for label-free study of bio-molecular interactions in real time. In a typical SPR experiment, one component (usually a protein, termed 'ligand') is immobilized onto a sensor chip surface, while the other (the 'analyte') is free in solution and is injected over the surface. Association and dissociation of the analyte from the ligand are measured and plotted in real time on a graph called a sensogram, from which pre-equilibrium and equilibrium data is derived. Being label-free, consuming low amounts of material, and providing pre-equilibrium kinetic data, often makes SPR the method of choice when studying dynamics of protein interactions. However, one has to keep in mind that due to the method's high sensitivity, the data obtained needs to be carefully analyzed, and supported by other biochemical methods. SPR is particularly suitable for studying membrane proteins since it consumes small amounts of purified material, and is compatible with lipids and detergents. This protocol describes an SPR experiment characterizing the kinetic properties of the interaction between a membrane protein (an ABC transporter) and a soluble protein (the transporter's cognate substrate binding protein)." @default.
• W2086587029 created "2016-06-24" @default.
• W2086587029 creator A5002098153 @default.
• W2086587029 creator A5005965754 @default.
• W2086587029 creator A5047588285 @default.
• W2086587029 date "2014-11-29" @default.
• W2086587029 modified "2023-10-16" @default.
• W2086587029 title "Real Time Measurements of Membrane Protein:Receptor Interactions Using Surface Plasmon Resonance (SPR)" @default.
• W2086587029 cites W1490146717 @default.
• W2086587029 cites W1980897131 @default.
• W2086587029 cites W2043157066 @default.
• W2086587029 cites W2063047484 @default.
• W2086587029 cites W2064059972 @default.
• W2086587029 cites W2071356190 @default.
• W2086587029 cites W2080085249 @default.
• W2086587029 cites W2137596950 @default.
• W2086587029 cites W2148091352 @default.
• W2086587029 cites W54085521 @default.
• W2086587029 doi "https://doi.org/10.3791/51937" @default.
• W2086587029 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4354417" @default.
• W2086587029 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25489923" @default.
• W2086587029 hasPublicationYear "2014" @default.
• W2086587029 type Work @default.
• W2086587029 sameAs 2086587029 @default.
• W2086587029 citedByCount "6" @default.
• W2086587029 countsByYear W20865870292016 @default.
• W2086587029 countsByYear W20865870292017 @default.
• W2086587029 countsByYear W20865870292019 @default.
• W2086587029 countsByYear W20865870292021 @default.
• W2086587029 crossrefType "journal-article" @default.
• W2086587029 hasAuthorship W2086587029A5002098153 @default.
• W2086587029 hasAuthorship W2086587029A5005965754 @default.
• W2086587029 hasAuthorship W2086587029A5047588285 @default.
• W2086587029 hasBestOaLocation W20865870292 @default.
• W2086587029 hasConcept C102931765 @default.
• W2086587029 hasConcept C10390740 @default.
• W2086587029 hasConcept C104317684 @default.
• W2086587029 hasConcept C106847996 @default.
• W2086587029 hasConcept C113196181 @default.
• W2086587029 hasConcept C11804247 @default.
• W2086587029 hasConcept C12554922 @default.
• W2086587029 hasConcept C144647389 @default.
• W2086587029 hasConcept C147789679 @default.
• W2086587029 hasConcept C149011108 @default.
• W2086587029 hasConcept C150194340 @default.
• W2086587029 hasConcept C155672457 @default.
• W2086587029 hasConcept C160756335 @default.
• W2086587029 hasConcept C170493617 @default.
• W2086587029 hasConcept C171250308 @default.
• W2086587029 hasConcept C185592680 @default.
• W2086587029 hasConcept C186060115 @default.
• W2086587029 hasConcept C192562407 @default.
• W2086587029 hasConcept C2910703447 @default.
• W2086587029 hasConcept C41625074 @default.
• W2086587029 hasConcept C43617362 @default.
• W2086587029 hasConcept C55493867 @default.
• W2086587029 hasConcept C74998103 @default.
• W2086587029 hasConcept C86803240 @default.
• W2086587029 hasConcept C95371953 @default.
• W2086587029 hasConceptScore W2086587029C102931765 @default.
• W2086587029 hasConceptScore W2086587029C10390740 @default.
• W2086587029 hasConceptScore W2086587029C104317684 @default.
• W2086587029 hasConceptScore W2086587029C106847996 @default.
• W2086587029 hasConceptScore W2086587029C113196181 @default.
• W2086587029 hasConceptScore W2086587029C11804247 @default.
• W2086587029 hasConceptScore W2086587029C12554922 @default.
• W2086587029 hasConceptScore W2086587029C144647389 @default.
• W2086587029 hasConceptScore W2086587029C147789679 @default.
• W2086587029 hasConceptScore W2086587029C149011108 @default.
• W2086587029 hasConceptScore W2086587029C150194340 @default.
• W2086587029 hasConceptScore W2086587029C155672457 @default.
• W2086587029 hasConceptScore W2086587029C160756335 @default.
• W2086587029 hasConceptScore W2086587029C170493617 @default.
• W2086587029 hasConceptScore W2086587029C171250308 @default.
• W2086587029 hasConceptScore W2086587029C185592680 @default.
• W2086587029 hasConceptScore W2086587029C186060115 @default.
• W2086587029 hasConceptScore W2086587029C192562407 @default.
• W2086587029 hasConceptScore W2086587029C2910703447 @default.
• W2086587029 hasConceptScore W2086587029C41625074 @default.
• W2086587029 hasConceptScore W2086587029C43617362 @default.
• W2086587029 hasConceptScore W2086587029C55493867 @default.
• W2086587029 hasConceptScore W2086587029C74998103 @default.
• W2086587029 hasConceptScore W2086587029C86803240 @default.
• W2086587029 hasConceptScore W2086587029C95371953 @default.
• W2086587029 hasIssue "93" @default.
• W2086587029 hasLocation W20865870291 @default.
• W2086587029 hasLocation W20865870292 @default.
• W2086587029 hasLocation W20865870293 @default.
• W2086587029 hasLocation W20865870294 @default.
• W2086587029 hasOpenAccess W2086587029 @default.
• W2086587029 hasPrimaryLocation W20865870291 @default.
• W2086587029 hasRelatedWork W1174728890 @default.
• W2086587029 hasRelatedWork W1992413341 @default.
• W2086587029 hasRelatedWork W2028067413 @default.
• W2086587029 hasRelatedWork W2038993805 @default.
• W2086587029 hasRelatedWork W2086587029 @default.
• W2086587029 hasRelatedWork W2169464969 @default.
• W2086587029 hasRelatedWork W2375482241 @default.
• W2086587029 hasRelatedWork W3197320732 @default.
• W2086587029 hasRelatedWork W4292883403 @default.

• next