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- W2097788589 abstract "This paper describes expression profiles of the Arabidopsis HSP18.2 heat-shock gene promoter by using three different reporter genes, and the application of this promoter to a method we have developed to drive heat-shock-dependent transcription of plant genome DNA flanked by T-DNA insertions. We show that, irrespective of the location of the T-DNA insertion, an HSP18.2 promoter towards the left border of the T-DNA effectively induces transcription of flanking genome sequences in response to heat shock. If polyadenylated, tagged transcripts can be easily identified by RT-PCR." @default.
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- W2097788589 date "2000-04-01" @default.
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- W2097788589 title "Heat-shock tagging: a simple method for expression and isolation of plant genome DNA flanked by T-DNA insertions" @default.
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- W2097788589 doi "https://doi.org/10.1046/j.1365-313x.2000.00716.x" @default.
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