FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2100072622> ?p ?o ?g. }

• W2100072622 endingPage "3552" @default.
• W2100072622 startingPage "3542" @default.
• W2100072622 abstract "The modification of proteins by nonenzymatic glycation leading to accumulation of advanced glycation end products (AGEs) is a well-established phenomenon of aging. In the eyes of elderly patients, these adducts have been observed in retinal pigment epithelium (RPE), particularly within the underlying pentalaminar substrate known as Bruch's membrane. AGEs have also been localized to age-related subcellular deposits (drusen and basal laminar deposits) and are thought to play a pathogenic role in progression of the major sight-threatening condition known as age-related macular degeneration (AMD). The current study has quantified AGEs in Bruch's membrane from postmortem eyes and established age-related correlations. In particular, we investigated the potential of confocal Raman microscopy to identify and quantify AGEs in Bruch's membrane in a nondestructive, analytical fashion. Bruch's membrane and the innermost layers of the underlying choroid (BM-Ch) were dissected from fresh postmortem eye-cups (n=56). AGE adducts were quantified from homogenized tissue using reverse-phase HPLC and GC/MS in combination with immunohistochemistry. For parallel Raman analysis, BM-Ch was flat-mounted on slides and evaluated using a Raman confocal microscope and spectra analyzed by a range of statistical approaches. Quantitative analysis showed that the AGEs pentosidine, carboxymethyllysine (CML), and carboxyethyllysine (CEL) occurred at significantly higher levels in BM-Ch with age (P<0.05-0.01). Defined Raman spectral fingerprints were identified for various AGEs and these were observed in the clinical samples using confocal Raman microscopy. The Raman data set successfully modeled AGEs and not only provided quantitative data that compared with conventional analytical approaches, but also provided new and complementary information via a nondestructive approach with high spatial resolution. It was shown that the Raman approach could be used to predict chronological age of the clinical samples (P<0.001) and a difference in the Raman spectra between genders was highly significant (P<0.000001). With further development, this Raman-based approach has the potential for noninvasive examination of AGE adducts in living eyes and ultimately to assess their precise pathogenic role in age-related diseases." @default.
• W2100072622 created "2016-06-24" @default.
• W2100072622 creator A5025321891 @default.
• W2100072622 creator A5041600049 @default.
• W2100072622 creator A5044963178 @default.
• W2100072622 creator A5059483104 @default.
• W2100072622 creator A5076757060 @default.
• W2100072622 creator A5080522053 @default.
• W2100072622 creator A5082728845 @default.
• W2100072622 creator A5087140822 @default.
• W2100072622 date "2007-06-12" @default.
• W2100072622 modified "2023-10-16" @default.
• W2100072622 title "Confocal Raman microscopy can quantify advanced glycation end product (AGE) modifications in Bruch's membrane leading to accurate, nondestructive prediction of ocular aging" @default.
• W2100072622 cites W1512942014 @default.
• W2100072622 cites W1799724127 @default.
• W2100072622 cites W1896619220 @default.
• W2100072622 cites W1966527137 @default.
• W2100072622 cites W1967665222 @default.
• W2100072622 cites W1978793468 @default.
• W2100072622 cites W1982698379 @default.
• W2100072622 cites W1985775184 @default.
• W2100072622 cites W1989822993 @default.
• W2100072622 cites W1991840137 @default.
• W2100072622 cites W1993397750 @default.
• W2100072622 cites W2000671659 @default.
• W2100072622 cites W2001507534 @default.
• W2100072622 cites W2004058656 @default.
• W2100072622 cites W2007804077 @default.
• W2100072622 cites W2014881030 @default.
• W2100072622 cites W2015678585 @default.
• W2100072622 cites W2034751842 @default.
• W2100072622 cites W2044712549 @default.
• W2100072622 cites W2046492603 @default.
• W2100072622 cites W2047028621 @default.
• W2100072622 cites W2052681712 @default.
• W2100072622 cites W2054221638 @default.
• W2100072622 cites W2057462051 @default.
• W2100072622 cites W2057862627 @default.
• W2100072622 cites W2059117797 @default.
• W2100072622 cites W2059277692 @default.
• W2100072622 cites W2080925629 @default.
• W2100072622 cites W2096528326 @default.
• W2100072622 cites W2107516773 @default.
• W2100072622 cites W2114283061 @default.
• W2100072622 cites W2114837987 @default.
• W2100072622 cites W2133022777 @default.
• W2100072622 cites W2146456294 @default.
• W2100072622 cites W2153605160 @default.
• W2100072622 cites W2155236757 @default.
• W2100072622 cites W2159404941 @default.
• W2100072622 cites W2163425767 @default.
• W2100072622 cites W2165322217 @default.
• W2100072622 cites W3024383408 @default.
• W2100072622 doi "https://doi.org/10.1096/fj.06-7896com" @default.
• W2100072622 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17567569" @default.
• W2100072622 hasPublicationYear "2007" @default.
• W2100072622 type Work @default.
• W2100072622 sameAs 2100072622 @default.
• W2100072622 citedByCount "102" @default.
• W2100072622 countsByYear W21000726222012 @default.
• W2100072622 countsByYear W21000726222013 @default.
• W2100072622 countsByYear W21000726222014 @default.
• W2100072622 countsByYear W21000726222015 @default.
• W2100072622 countsByYear W21000726222016 @default.
• W2100072622 countsByYear W21000726222017 @default.
• W2100072622 countsByYear W21000726222018 @default.
• W2100072622 countsByYear W21000726222019 @default.
• W2100072622 countsByYear W21000726222020 @default.
• W2100072622 countsByYear W21000726222021 @default.
• W2100072622 countsByYear W21000726222022 @default.
• W2100072622 crossrefType "journal-article" @default.
• W2100072622 hasAuthorship W2100072622A5025321891 @default.
• W2100072622 hasAuthorship W2100072622A5041600049 @default.
• W2100072622 hasAuthorship W2100072622A5044963178 @default.
• W2100072622 hasAuthorship W2100072622A5059483104 @default.
• W2100072622 hasAuthorship W2100072622A5076757060 @default.
• W2100072622 hasAuthorship W2100072622A5080522053 @default.
• W2100072622 hasAuthorship W2100072622A5082728845 @default.
• W2100072622 hasAuthorship W2100072622A5087140822 @default.
• W2100072622 hasConcept C118487528 @default.
• W2100072622 hasConcept C120665830 @default.
• W2100072622 hasConcept C121332964 @default.
• W2100072622 hasConcept C136009344 @default.
• W2100072622 hasConcept C142724271 @default.
• W2100072622 hasConcept C147990577 @default.
• W2100072622 hasConcept C169760540 @default.
• W2100072622 hasConcept C170493617 @default.
• W2100072622 hasConcept C185592680 @default.
• W2100072622 hasConcept C2776403814 @default.
• W2100072622 hasConcept C2776582655 @default.
• W2100072622 hasConcept C2776694393 @default.
• W2100072622 hasConcept C2776997535 @default.
• W2100072622 hasConcept C2777093970 @default.
• W2100072622 hasConcept C2778024200 @default.
• W2100072622 hasConcept C2779093074 @default.
• W2100072622 hasConcept C2779178360 @default.
• W2100072622 hasConcept C2780827179 @default.
• W2100072622 hasConcept C40003534 @default.

• next