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• W2108374461 abstract "Migratory cells, like mammalian leukocytes and Dictyostelium, utilize G protein coupled receptor (GPCR) signaling to regulate MAPK/ERK, PI3K, TORC2/AKT, adenylyl cyclase, and actin polymerization, which collectively direct chemotaxis. Upon ligand binding, mammalian GPCRs are phosphorylated at cytoplasmic residues, uncoupling G protein pathways, but activating others. Still, connections between GPCR phosphorylation and chemotaxis are unclear. In developing Dictyostelium, secreted cAMP serves as a chemoattractant, with extracellular cAMP propagated as oscillating waves to ensure directional migratory signals. cAMP oscillations derive from transient excitatory responses of adenylyl cyclase, which then rapidly adapts. We have studied chemotactic signaling in Dictyostelium that express non-phosphorylatable cAMP receptors and show through chemotaxis modeling, single-cell FRET imaging, pure and chimeric population wavelet quantification, biochemical analyses, and TIRF microscopy, that receptor phosphorylation is required to regulate adenylyl cyclase adaptation, long-range oscillatory cAMP wave production, and cytoskeletal actin response. Phosphorylation defects, thus, promote hyperactive actin polymerization at the cell periphery, misdirected pseudopodia, and the loss of directional chemotaxis. Our data indicate that chemoattractant receptor phosphorylation is required to co-regulate essential pathways for migratory cell polarization and chemotaxis. Our results significantly extend the understanding of GPCR phosphorylation function, providing strong evidence that this evolutionarily conserved mechanism is required in a signal attenuation pathway that is necessary to maintain persistent directional movement of Dictyostelium, neutrophils, and other migratory cells." @default.
• W2108374461 created "2016-06-24" @default.
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• W2108374461 creator A5082247385 @default.
• W2108374461 date "2013-01-01" @default.
• W2108374461 modified "2023-10-16" @default.
• W2108374461 title "Phosphorylation of chemoattractant receptors regulates chemotaxis, actin re-organization, and signal-relay" @default.
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• W2108374461 doi "https://doi.org/10.1242/jcs.122952" @default.
• W2108374461 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3795335" @default.
• W2108374461 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23902692" @default.
• W2108374461 hasPublicationYear "2013" @default.
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