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• W2109492080 abstract "A simple, rapid, and user-friendly procedure has been developed to identify Staphylococcus aureus and determine its methicillin resistance directly from gram-positive cocci in cluster-containing blood culture medium. The specimens were diluted and heated prior to amplification of the nuc and mecA genes with isothermal helicase-dependent amplification. Amplicons were detected using a disposable detection device. The analytical sensitivity of the assays was 50 CFU per reaction, and the clinical sensitivity and specificity were both 100% for S. aureus detection and 100% and 98% for methicillin resistance determination, respectively." @default.
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• W2109492080 date "2008-04-01" @default.
• W2109492080 modified "2023-10-16" @default.
• W2109492080 title "Identification of <i>Staphylococcus aureus</i> and Determination of Methicillin Resistance Directly from Positive Blood Cultures by Isothermal Amplification and a Disposable Detection Device" @default.
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• W2109492080 doi "https://doi.org/10.1128/jcm.02234-07" @default.
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