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- W2112924439 abstract "Fluorescence microscopy is indispensable in nanoscience and biological sciences. The versatility of labeling target structures with fluorescent dyes permits to visualize structure and function at a subcellular resolution with a wide field of view. Due to the diffraction limit, conventional optical microscopes are limited to resolving structures larger than 200 nm. The resolution can be enhanced by near-field and far-field super-resolution microscopy methods. Near-field methods typically have a limited field of view and far-field methods are limited by the involved conventional optics. Here, we introduce a combined high-resolution and wide-field fluorescence microscopy method that improves the resolution of a conventional optical microscope by exploiting correlations in speckle illumination through a randomly scattering high-index medium: Speckle correlation resolution enhancement (SCORE). As a test, we collect two-dimensional fluorescence images of 100-nm diameter dye-doped nanospheres. We demonstrate a deconvolved resolution of 130 nm with a field of view of 10 x 10 $text{mu m}^2$." @default.
- W2112924439 created "2016-06-24" @default.
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- W2112924439 date "2015-04-27" @default.
- W2112924439 modified "2023-09-30" @default.
- W2112924439 title "Speckle correlation resolution enhancement of wide-field fluorescence imaging" @default.
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- W2112924439 doi "https://doi.org/10.1364/optica.2.000424" @default.
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