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• W2113386151 abstract "Protein extraction is a crucial step for proteomics studies. To establish an effective protein extraction protocol suitable for two‐dimensional electrophoresis (2 DE ) analysis in J erusalem artichoke ( H elianthus tuberosus L .), three different protein extraction methods—trichloroacetic acid/acetone, M g/ NP ‐40, and phenol/ammonium acetate—were evaluated using J erusalem artichoke leaves as source materials. Of the three methods, trichloroacetic acid/acetone yielded the best protein separation pattern and highest number of protein spots in 2 DE analysis. Proteins highly abundant in leaves, such as R ubisco, are typically problematic during leaf 2 DE analysis, however, and this disadvantage was evident using trichloroacetic acid/acetone. To reduce the influence of abundant proteins on the detection of low‐abundance proteins, we optimized the trichloroacetic acid/acetone method by incorporating a PEG fractionation approach. After optimization, 363 additional (36.2%) protein spots were detected on the 2 DE gel. Our results suggest that trichloroacetic acid/acetone method is a better protein extraction technique than M g/ NP ‐40 and phenol/ammonium acetate in J erusalem artichoke leaf 2 DE analysis, and that trichloroacetic acid/acetone method combined with PEG fractionation procedure is the most effective approach for leaf 2 DE analysis of Jerusalem artichoke." @default.
• W2113386151 created "2016-06-24" @default.
• W2113386151 creator A5048612387 @default.
• W2113386151 creator A5066887412 @default.
• W2113386151 date "2013-06-10" @default.
• W2113386151 modified "2023-10-04" @default.
• W2113386151 title "Effective protein extraction protocol for proteomics studies of Jerusalem artichoke leaves" @default.
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• W2113386151 doi "https://doi.org/10.1002/jssc.201300199" @default.
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