FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2116732994> ?p ?o ?g. }

• W2116732994 endingPage "16" @default.
• W2116732994 startingPage "1109" @default.
• W2116732994 abstract "The objective of this study was to develop and evaluate a primary culture system for choroid plexus epithelial cells as an in vitro model for studying organic cation transport. Cells were dispersed from choroid plexus of neonatal rats by enzymatic digestion and grew as differentiated monolayers when plated on solid or permeable support. Electron microscopy showed that cultured cells were morphologically similar to intact choroid plexus epithelium, having apical tight junctions between cells, numerous mitochondria, basal nuclei and apical microvilli and cilia. As previously demonstrated for intact choroid plexus, immunocytochemistry showed that Na+,K+-ATPase was localized to the apical membrane, and GLUT-1, the facilitative glucose transporter, was localized to the basolateral membrane of cultured cells. Apical transport of L-proline by cultured cells was mediated by a sodium-dependent, electrogenic process, as in whole tissue. 14C-Tetraethylammonium (TEA), a prototypic organic cation, was accumulated by isolated choroid plexus in a time-dependent manner; uptake was inhibited by tetrapentylammonium (TePA). In cultured cells, apical TEA transport was mediated by a saturable process coupled to cellular metabolism. Unlabeled TEA and other organic cations (TePA, N1-methylnicotinamide and mepiperphenidol) inhibited TEA transport; the organic anion, p-aminohippurate, had no effect. Finally, TePA-sensitive transport of 14C-TEA was stimulated after preloading the cells with unlabeled TEA. Based on the morphological, biochemical and functional properties of these cultured cells, we conclude that this primary culture system should be an excellent in vitro model for experimental characterization of choroid plexus function." @default.
• W2116732994 created "2016-06-24" @default.
• W2116732994 creator A5032465432 @default.
• W2116732994 creator A5069632633 @default.
• W2116732994 creator A5073906106 @default.
• W2116732994 date "1997-08-01" @default.
• W2116732994 modified "2023-09-23" @default.
• W2116732994 title "Functional characterization of choroid plexus epithelial cells in primary culture." @default.
• W2116732994 cites W1480192557 @default.
• W2116732994 cites W1797273806 @default.
• W2116732994 cites W1858703719 @default.
• W2116732994 cites W1971983756 @default.
• W2116732994 cites W1972324674 @default.
• W2116732994 cites W1972712399 @default.
• W2116732994 cites W1985265289 @default.
• W2116732994 cites W1985274228 @default.
• W2116732994 cites W1985637387 @default.
• W2116732994 cites W1992157714 @default.
• W2116732994 cites W1996740012 @default.
• W2116732994 cites W2001060570 @default.
• W2116732994 cites W2007237513 @default.
• W2116732994 cites W2013063312 @default.
• W2116732994 cites W2015614939 @default.
• W2116732994 cites W2024312881 @default.
• W2116732994 cites W2031438971 @default.
• W2116732994 cites W2036159637 @default.
• W2116732994 cites W2073196712 @default.
• W2116732994 cites W2078244250 @default.
• W2116732994 cites W2085790414 @default.
• W2116732994 cites W2110799600 @default.
• W2116732994 cites W2141244106 @default.
• W2116732994 cites W2147463574 @default.
• W2116732994 cites W2148973531 @default.
• W2116732994 cites W2150307142 @default.
• W2116732994 cites W2207879974 @default.
• W2116732994 cites W2222076283 @default.
• W2116732994 cites W2268207664 @default.
• W2116732994 cites W2284699406 @default.
• W2116732994 cites W2292012697 @default.
• W2116732994 cites W2298286985 @default.
• W2116732994 cites W2312337351 @default.
• W2116732994 cites W2335803388 @default.
• W2116732994 cites W2399926305 @default.
• W2116732994 cites W2409784947 @default.
• W2116732994 cites W2419322002 @default.
• W2116732994 cites W298714662 @default.
• W2116732994 cites W99164418 @default.
• W2116732994 cites W1857779262 @default.
• W2116732994 cites W2016996076 @default.
• W2116732994 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9262381" @default.
• W2116732994 hasPublicationYear "1997" @default.
• W2116732994 type Work @default.
• W2116732994 sameAs 2116732994 @default.
• W2116732994 citedByCount "21" @default.
• W2116732994 countsByYear W21167329942014 @default.
• W2116732994 countsByYear W21167329942015 @default.
• W2116732994 countsByYear W21167329942017 @default.
• W2116732994 countsByYear W21167329942022 @default.
• W2116732994 crossrefType "journal-article" @default.
• W2116732994 hasAuthorship W2116732994A5032465432 @default.
• W2116732994 hasAuthorship W2116732994A5069632633 @default.
• W2116732994 hasAuthorship W2116732994A5073906106 @default.
• W2116732994 hasConcept C104317684 @default.
• W2116732994 hasConcept C12554922 @default.
• W2116732994 hasConcept C134018914 @default.
• W2116732994 hasConcept C149011108 @default.
• W2116732994 hasConcept C1491633281 @default.
• W2116732994 hasConcept C153911025 @default.
• W2116732994 hasConcept C178790620 @default.
• W2116732994 hasConcept C185592680 @default.
• W2116732994 hasConcept C189613389 @default.
• W2116732994 hasConcept C202751555 @default.
• W2116732994 hasConcept C2776538271 @default.
• W2116732994 hasConcept C2781295685 @default.
• W2116732994 hasConcept C3018436504 @default.
• W2116732994 hasConcept C35866371 @default.
• W2116732994 hasConcept C39587587 @default.
• W2116732994 hasConcept C41625074 @default.
• W2116732994 hasConcept C517785266 @default.
• W2116732994 hasConcept C529278444 @default.
• W2116732994 hasConcept C529295009 @default.
• W2116732994 hasConcept C54355233 @default.
• W2116732994 hasConcept C55493867 @default.
• W2116732994 hasConcept C86803240 @default.
• W2116732994 hasConcept C95444343 @default.
• W2116732994 hasConceptScore W2116732994C104317684 @default.
• W2116732994 hasConceptScore W2116732994C12554922 @default.
• W2116732994 hasConceptScore W2116732994C134018914 @default.
• W2116732994 hasConceptScore W2116732994C149011108 @default.
• W2116732994 hasConceptScore W2116732994C1491633281 @default.
• W2116732994 hasConceptScore W2116732994C153911025 @default.
• W2116732994 hasConceptScore W2116732994C178790620 @default.
• W2116732994 hasConceptScore W2116732994C185592680 @default.
• W2116732994 hasConceptScore W2116732994C189613389 @default.
• W2116732994 hasConceptScore W2116732994C202751555 @default.
• W2116732994 hasConceptScore W2116732994C2776538271 @default.
• W2116732994 hasConceptScore W2116732994C2781295685 @default.
• W2116732994 hasConceptScore W2116732994C3018436504 @default.

• next