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• W2116739202 abstract "Swine beta interferon (swIFN- β ) produced in HEK 293 cells infected with a recombinant, replication-defective human adenovirus 5 (Ad5) encoding the swIFN- β gene was tested for antiviral activity against Porcine reproductive and respiratory syndrome virus (PRRSV). MARC-145 cells were incubated overnight with dilutions of supernatant fluids from HEK 293 cells infected with Ad5-swIFN- β or with an Ad5 control virus (Ad5-Blue). Treated cells were infected with PRRSV; MARC-145 cells incubated with Ad5-Blue supernatants developed cytopathic effects (CPE), whereas those incubated with swIFN- β showed no CPE. To confirm the antiviral activity of swIFN- β , culture fluids from Ad5-swIFN- β -infected cells were affinity-purified on a Sepharose–anti-swIFN- β matrix, and the resulting fractions exhibited antiviral activity upon infection with PRRSV. The antiviral effects were specific, as they were blocked by mAbs against swIFN- β . Additional cultures of MARC-145 cells treated with swIFN- β -containing supernatants or affinity-purified swIFN- β were infected with PRRSV and tested by real-time RT-PCR for viral RNA in culture supernatants at various times post-inoculation. These experiments confirmed the protective effects of swIFN- β . swIFN- β was also tested for antiviral activity on porcine alveolar macrophages (PAMs) obtained by bronchoalveolar lavage from PRRSV-negative swine. PAMs were treated with dilutions of swIFN- β or Ad5-Blue culture fluids, infected with PRRSV and tested for viral RNA by real-time RT-PCR. The viral load data showed a dose-dependent protection in swIFN- β -treated PAMs, whereas no protection was evident from Ad5-Blue culture fluids. The data demonstrate that swIFN- β protects both MARC-145 cells and PAMs from PRRSV infection." @default.
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• W2116739202 date "2007-03-01" @default.
• W2116739202 modified "2023-10-18" @default.
• W2116739202 title "Recombinant swine beta interferon protects swine alveolar macrophages and MARC-145 cells from infection with Porcine reproductive and respiratory syndrome virus" @default.
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• W2116739202 doi "https://doi.org/10.1099/vir.0.82585-0" @default.
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