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• W2116795432 abstract "Abstract Eukaryotic origins of DNA replication undergo activation at various times in S-phase, allowing the genome to be duplicated in a temporally staggered fashion. In the budding yeast Saccharomyces cerevisiae, the activation times of individual origins are not intrinsic to those origins but are instead governed by surrounding sequences. Currently, there are two examples of DNA sequences that are known to advance origin activation time, centromeres and forkhead transcription factor binding sites. By combining deletion and linker scanning mutational analysis with two-dimensional gel electrophoresis to measure fork direction in the context of a two-origin plasmid, we have identified and characterized a 19- to 23-bp and a larger 584-bp DNA sequence that are capable of advancing origin activation time." @default.
• W2116795432 created "2016-06-24" @default.
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• W2116795432 date "2013-11-01" @default.
• W2116795432 modified "2023-09-23" @default.
• W2116795432 title "A DNA Sequence Element That Advances Replication Origin Activation Time in <i>Saccharomyces cerevisiae</i>" @default.
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• W2116795432 doi "https://doi.org/10.1534/g3.113.008250" @default.
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