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• W2116796181 endingPage "84" @default.
• W2116796181 startingPage "79" @default.
• W2116796181 abstract "Interactions of 5,10,15,20-Tetrakis(N-methylpyridinium-4-yl)-21H,23H-porphyrin (TMPyP4) and 5,10,15,20-Tetrakis(N-propylpyridinium-4-yl)-21H,23H-porphyrin (TPrPyP4) with the parallel four-stranded (TG(4)T)4 G-quadruplex DNA in 100 mM K(+)-containing buffer were studied using circular dichroism (CD) spectroscopy, visible absorption titration, and steady and time-resolved fluorescence spectroscopies. The results show that the binding stoichiometric ratios of both TMPyP4 and TPrPyP4 to (TG(4)T)4 are 3:1. Two types of independent and nonequivalent binding sites with the higher and lower binding affinities are confirmed, and the stronger and weaker binding constants are 9.44x10(7) and 6.94x10(5) M(-1) for (TG(4)T)4-TMPyP4 complex, 7.86x10(7) and 6.35x10(5) M(-1) for (TG(4)T)4-TPrPyP4 complex, respectively. For both TMPyP4-(TG(4)T)4 and TPrPyP4-(TG(4)T)4 complexes, one porphyrin molecule stacks on the one end of G-quadruplex with the higher binding affinity, another two porphyrins bind weakly to the two external grooves. The size of cation side arms around porphyrin core almost fails to affect the binding mode, stoichiometry and affinity of porphyrin to (TG(4)T)4 G-quadruplex in 100 mM K(+)-containing buffer." @default.
• W2116796181 created "2016-06-24" @default.
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• W2116796181 date "2009-07-01" @default.
• W2116796181 modified "2023-10-10" @default.
• W2116796181 title "The binding mode of porphyrins with cation side arms to (TG4T)4 G-quadruplex: Spectroscopic evidence" @default.
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• W2116796181 doi "https://doi.org/10.1016/j.bpc.2009.04.005" @default.
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