FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2122802199> ?p ?o ?g. }

• W2122802199 abstract "Human endonucleases are integral to apoptosis in which unwanted or potentially harmful cells are eliminated. The rapid turnover of ocular surface epithelium and microbial colonization of the eyelids are continual sources of DNA in tears. Here, we determine the principal sources of endonuclease activity in tears.Endonucleases in human tears were identified after Sephadex G100 gel filtration. DNA hydrolyzing activity was measured by the conversion pUC19 plasmid DNA to its circular form in agarose gels. Fractions with endonuclease activity were further isolated using a combination ConA-Sepharose DNA, oligo (dT) cellulose, and anion exchange chromatographies. The molecular weights of the DNA hydrolyzing proteins were estimated in zymograms and by calibration of size exclusion chromatography. DNase activities were characterized for activity at a variety of pH and ion concentrations as well as in the presence of inhibitors including NiCl(2), ZnCl(2), G-actin, and aurintricarboxylic acid (ATA). To determine the mode of hydrolysis, the cleaved ends of the DNA digested by tear DNases were analyzed by 3' and 5' end labeling using either terminal deoxynucleotidyl transferase or polynucleotide kinase with or without pretreatment with alkaline phosphatase.Tear lipocalin (TL) accounts for over 75% of the DNA catalytic activity in tears while a second endonuclease, approximately 34 kDa, is responsible for less than 24% of the activity. Both are Mg(2+) dependent enzyme endonucleases that are enhanced by Ca(2+), active at physiologic pH, inhibited by aurintricarboxylic acid, and catalyze hydrolysis of DNA to produce 3'-OH/5'P ends. However, the two enzymes can be distinguished by the inhibitory effect of NiCl(2) and the sizes of the cleaved DNA fragments.Two magnesium dependent extracellular endonucleases were identified in tears that are different from other major human extracellular nucleases. TL is the principal endonuclease in human tear fluid. Tear endonucleases have unique characteristics that differ from other known human endonucleases." @default.
• W2122802199 created "2016-06-24" @default.
• W2122802199 creator A5021257902 @default.
• W2122802199 creator A5023818001 @default.
• W2122802199 creator A5066558990 @default.
• W2122802199 creator A5071666147 @default.
• W2122802199 creator A5086882823 @default.
• W2122802199 date "2008-01-29" @default.
• W2122802199 modified "2023-09-27" @default.
• W2122802199 title "Tear lipocalin is the major endonuclease in tears." @default.
• W2122802199 cites W1514713533 @default.
• W2122802199 cites W1567114372 @default.
• W2122802199 cites W1633463671 @default.
• W2122802199 cites W1891808122 @default.
• W2122802199 cites W1966515702 @default.
• W2122802199 cites W1968350013 @default.
• W2122802199 cites W1970349953 @default.
• W2122802199 cites W1979492674 @default.
• W2122802199 cites W1985132587 @default.
• W2122802199 cites W2009821572 @default.
• W2122802199 cites W2010004736 @default.
• W2122802199 cites W2031499346 @default.
• W2122802199 cites W2040228918 @default.
• W2122802199 cites W2042551883 @default.
• W2122802199 cites W2044206659 @default.
• W2122802199 cites W2046140230 @default.
• W2122802199 cites W2046231714 @default.
• W2122802199 cites W2046378458 @default.
• W2122802199 cites W2055523705 @default.
• W2122802199 cites W2056932865 @default.
• W2122802199 cites W2066378634 @default.
• W2122802199 cites W2077439976 @default.
• W2122802199 cites W2087224839 @default.
• W2122802199 cites W2091058610 @default.
• W2122802199 cites W2091210743 @default.
• W2122802199 cites W2112727307 @default.
• W2122802199 cites W2117934735 @default.
• W2122802199 cites W2152873202 @default.
• W2122802199 cites W2157539783 @default.
• W2122802199 cites W2409292833 @default.
• W2122802199 cites W2413849412 @default.
• W2122802199 cites W2442926998 @default.
• W2122802199 cites W379674329 @default.
• W2122802199 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2254967" @default.
• W2122802199 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18334931" @default.
• W2122802199 hasPublicationYear "2008" @default.
• W2122802199 type Work @default.
• W2122802199 sameAs 2122802199 @default.
• W2122802199 citedByCount "9" @default.
• W2122802199 countsByYear W21228021992012 @default.
• W2122802199 countsByYear W21228021992018 @default.
• W2122802199 countsByYear W21228021992020 @default.
• W2122802199 countsByYear W21228021992021 @default.
• W2122802199 countsByYear W21228021992022 @default.
• W2122802199 countsByYear W21228021992023 @default.
• W2122802199 crossrefType "journal-article" @default.
• W2122802199 hasAuthorship W2122802199A5021257902 @default.
• W2122802199 hasAuthorship W2122802199A5023818001 @default.
• W2122802199 hasAuthorship W2122802199A5066558990 @default.
• W2122802199 hasAuthorship W2122802199A5071666147 @default.
• W2122802199 hasAuthorship W2122802199A5086882823 @default.
• W2122802199 hasConcept C190283241 @default.
• W2122802199 hasConcept C20299238 @default.
• W2122802199 hasConcept C203014093 @default.
• W2122802199 hasConcept C2777028655 @default.
• W2122802199 hasConcept C2778275304 @default.
• W2122802199 hasConcept C54355233 @default.
• W2122802199 hasConcept C552990157 @default.
• W2122802199 hasConcept C55493867 @default.
• W2122802199 hasConcept C86803240 @default.
• W2122802199 hasConcept C95444343 @default.
• W2122802199 hasConceptScore W2122802199C190283241 @default.
• W2122802199 hasConceptScore W2122802199C20299238 @default.
• W2122802199 hasConceptScore W2122802199C203014093 @default.
• W2122802199 hasConceptScore W2122802199C2777028655 @default.
• W2122802199 hasConceptScore W2122802199C2778275304 @default.
• W2122802199 hasConceptScore W2122802199C54355233 @default.
• W2122802199 hasConceptScore W2122802199C552990157 @default.
• W2122802199 hasConceptScore W2122802199C55493867 @default.
• W2122802199 hasConceptScore W2122802199C86803240 @default.
• W2122802199 hasConceptScore W2122802199C95444343 @default.
• W2122802199 hasLocation W21228021991 @default.
• W2122802199 hasOpenAccess W2122802199 @default.
• W2122802199 hasPrimaryLocation W21228021991 @default.
• W2122802199 hasRelatedWork W1507155737 @default.
• W2122802199 hasRelatedWork W1551942171 @default.
• W2122802199 hasRelatedWork W1807341246 @default.
• W2122802199 hasRelatedWork W1964579574 @default.
• W2122802199 hasRelatedWork W1967633172 @default.
• W2122802199 hasRelatedWork W1976172969 @default.
• W2122802199 hasRelatedWork W1985026772 @default.
• W2122802199 hasRelatedWork W1987544804 @default.
• W2122802199 hasRelatedWork W1995462409 @default.
• W2122802199 hasRelatedWork W2005178146 @default.
• W2122802199 hasRelatedWork W2031921252 @default.
• W2122802199 hasRelatedWork W2040228918 @default.
• W2122802199 hasRelatedWork W2046140230 @default.
• W2122802199 hasRelatedWork W2061968195 @default.
• W2122802199 hasRelatedWork W2084489983 @default.
• W2122802199 hasRelatedWork W2091427621 @default.

• next