FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2132645356> ?p ?o ?g. }

• W2132645356 endingPage "2512" @default.
• W2132645356 startingPage "2502" @default.
• W2132645356 abstract "ABSTRACT A bile salt hydrolase (BSH) was isolated from Bifidobacterium longum SBT2928, purified, and characterized. Furthermore, we describe for the first time cloning and analysis of the gene encoding BSH ( bsh ) in a member of the genus Bifidobacterium . The enzyme has a native molecular weight of 125,000 to 130,000 and a subunit molecular weight of 35,024, as determined from the deduced amino acid sequence, indicating that the enzyme is a tetramer. The pH optimum of B. longum BSH is between 5 and 7, and the temperature optimum is 40°C. The enzyme is strongly inhibited by thiol enzyme inhibitors, indicating that a Cys residue is likely to be involved in the catalytic reaction. The BSH of B. longum can hydrolyze all six major human bile salts and at least two animal bile salts. A slight preference for glycine-conjugated bile acids was detected based on both the specificity and the K m values. The nucleotide sequence of bsh was determined and used for homology studies, transcript analysis, and construction and analysis of various mutants. The levels of homology with BSH of other bacteria and with penicillin V acylase (PVA) of Bacillus sphaericus were high. On the basis of the similarity of BSH and PVA, whose crystal structure has been elucidated, BSH can be classified as an N-terminal nucleophile hydrolase with Cys as the N-terminal amino acid. This classification was confirmed by the fact that a Cys1Ala exchange by site-directed mutagenesis resulted in an inactive protein. Reverse transcription-PCR experiments revealed that bsh is part of an operon containing at least two genes, bsh and glnE (GlnE is glutamine synthetase adenylyltransferase). Two UV-induced BSH-negative mutants and one spontaneous BSH-negative mutant were isolated from B. longum SBT2928 cultures and characterized. These mutants had point mutations that inactivated bsh by premature termination, frameshift, or amino acid exchange." @default.
• W2132645356 created "2016-06-24" @default.
• W2132645356 creator A5024844426 @default.
• W2132645356 creator A5041852525 @default.
• W2132645356 creator A5042321088 @default.
• W2132645356 creator A5062330206 @default.
• W2132645356 date "2000-06-01" @default.
• W2132645356 modified "2023-10-10" @default.
• W2132645356 title "Bile Salt Hydrolase of <i>Bifidobacterium longum</i> —Biochemical and Genetic Characterization" @default.
• W2132645356 cites W1524169968 @default.
• W2132645356 cites W1533296893 @default.
• W2132645356 cites W1547278883 @default.
• W2132645356 cites W1578765392 @default.
• W2132645356 cites W1837857344 @default.
• W2132645356 cites W1916997903 @default.
• W2132645356 cites W1932052686 @default.
• W2132645356 cites W1935149946 @default.
• W2132645356 cites W1942690669 @default.
• W2132645356 cites W1970697372 @default.
• W2132645356 cites W1987799789 @default.
• W2132645356 cites W1991471459 @default.
• W2132645356 cites W1996216081 @default.
• W2132645356 cites W2006502556 @default.
• W2132645356 cites W2014453458 @default.
• W2132645356 cites W2014570129 @default.
• W2132645356 cites W2017998089 @default.
• W2132645356 cites W2021896492 @default.
• W2132645356 cites W2022454496 @default.
• W2132645356 cites W2026491313 @default.
• W2132645356 cites W2031104654 @default.
• W2132645356 cites W2051224854 @default.
• W2132645356 cites W2053219431 @default.
• W2132645356 cites W2055856479 @default.
• W2132645356 cites W2064998663 @default.
• W2132645356 cites W2073866269 @default.
• W2132645356 cites W2084693146 @default.
• W2132645356 cites W2097678114 @default.
• W2132645356 cites W2100837269 @default.
• W2132645356 cites W2101108802 @default.
• W2132645356 cites W2101167562 @default.
• W2132645356 cites W2105514811 @default.
• W2132645356 cites W2121893220 @default.
• W2132645356 cites W2137451156 @default.
• W2132645356 cites W2138879032 @default.
• W2132645356 cites W2139325759 @default.
• W2132645356 cites W2140544780 @default.
• W2132645356 cites W2148305363 @default.
• W2132645356 cites W2153496450 @default.
• W2132645356 cites W2154495926 @default.
• W2132645356 cites W2158714788 @default.
• W2132645356 cites W2161587852 @default.
• W2132645356 cites W312239447 @default.
• W2132645356 cites W49155368 @default.
• W2132645356 cites W832803006 @default.
• W2132645356 doi "https://doi.org/10.1128/aem.66.6.2502-2512.2000" @default.
• W2132645356 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/110569" @default.
• W2132645356 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10831430" @default.
• W2132645356 hasPublicationYear "2000" @default.
• W2132645356 type Work @default.
• W2132645356 sameAs 2132645356 @default.
• W2132645356 citedByCount "228" @default.
• W2132645356 countsByYear W21326453562012 @default.
• W2132645356 countsByYear W21326453562013 @default.
• W2132645356 countsByYear W21326453562014 @default.
• W2132645356 countsByYear W21326453562015 @default.
• W2132645356 countsByYear W21326453562016 @default.
• W2132645356 countsByYear W21326453562017 @default.
• W2132645356 countsByYear W21326453562018 @default.
• W2132645356 countsByYear W21326453562019 @default.
• W2132645356 countsByYear W21326453562020 @default.
• W2132645356 countsByYear W21326453562021 @default.
• W2132645356 countsByYear W21326453562022 @default.
• W2132645356 countsByYear W21326453562023 @default.
• W2132645356 crossrefType "journal-article" @default.
• W2132645356 hasAuthorship W2132645356A5024844426 @default.
• W2132645356 hasAuthorship W2132645356A5041852525 @default.
• W2132645356 hasAuthorship W2132645356A5042321088 @default.
• W2132645356 hasAuthorship W2132645356A5062330206 @default.
• W2132645356 hasBestOaLocation W21326453561 @default.
• W2132645356 hasConcept C100544194 @default.
• W2132645356 hasConcept C181199279 @default.
• W2132645356 hasConcept C185592680 @default.
• W2132645356 hasConcept C2776996202 @default.
• W2132645356 hasConcept C2780120296 @default.
• W2132645356 hasConcept C2780206646 @default.
• W2132645356 hasConcept C2780305828 @default.
• W2132645356 hasConcept C515207424 @default.
• W2132645356 hasConcept C55493867 @default.
• W2132645356 hasConcept C86803240 @default.
• W2132645356 hasConceptScore W2132645356C100544194 @default.
• W2132645356 hasConceptScore W2132645356C181199279 @default.
• W2132645356 hasConceptScore W2132645356C185592680 @default.
• W2132645356 hasConceptScore W2132645356C2776996202 @default.
• W2132645356 hasConceptScore W2132645356C2780120296 @default.
• W2132645356 hasConceptScore W2132645356C2780206646 @default.
• W2132645356 hasConceptScore W2132645356C2780305828 @default.
• W2132645356 hasConceptScore W2132645356C515207424 @default.
• W2132645356 hasConceptScore W2132645356C55493867 @default.

• next