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• W2163041776 abstract "The extracellular matrix (ECM), constituting the follicular basal lamina and present also between follicular cells and in the follicular fluid, is believed to regulate granulosa cell (GC) function during follicular development. Ovine GCs isolated from small (1-3 mm in diameter) or large (4-7 mm in diameter) antral follicles were cultured on various pure ECM components (type I collagen, fibronectin, laminin), synthetic substrata enhancing (RGD peptides) or impairing (poly 2-hydroxyethylmethacrylate (poly-hema)) cell adhesion, or in the presence of heparin. The effects of these factors, used alone or in combination with IGF-I and/or FSH, were evaluated in terms of GC spread, survival, proliferation and steroidogenesis. When grown on type I collagen (CI) gel, poly-hema or heparin, GCs from both large and small follicles exhibited a round shape and a low proliferation rate. Compared with non-coated plastic substratum as a control, these ECM or synthetic compounds enhanced estradiol secretion and reduced progesterone secretion by large-follicle GCs. In contrast, GCs from both large and small follicles spread extensively on CI coating, fibronectin, laminin and RGD peptides. Fibronectin and laminin dramatically increased the proliferation rate and enhanced survival of GCs from both origins. Moreover, fibronectin, laminin and RGD peptides reduced estradiol secretion by large-follicle GCs. Unexpectedly, CI coating increased estradiol secretion and reduced progesterone secretion by large-follicle GCs, suggesting that type I collagen was able to maintain estradiol secretion independently of GC shape. Finally, GC responsiveness to IGF-I and FSH, in terms of proliferation and steroidogenesis, was generally maintained when cells were grown on ECM components, RGD peptides and in the presence of heparin. However, when large-follicle GCs were grown as non-adherent clusters (as observed on poly-hema) basal and IGF-I- and/or FSH-stimulated progesterone secretions were totally abolished. Overall, this study shows that GC shape, survival, proliferation and steroidogenesis can be modulated in vitro by pure ECM components in a specific and coordinated manner. It is suggested that, in vivo, fibronectin and laminin would sustain follicular development by enhancing the survival and proliferation of GCs, whereas type I collagen might participate in the maintenance of estradiol secretion in large antral follicles." @default.
• W2163041776 created "2016-06-24" @default.
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• W2163041776 date "2001-05-01" @default.
• W2163041776 modified "2023-09-25" @default.
• W2163041776 title "Extracellular matrix regulates ovine granulosa cell survival, proliferation and steroidogenesis: relationships between cell shape and function" @default.
• W2163041776 cites W103200536 @default.
• W2163041776 cites W1500829200 @default.
• W2163041776 cites W1534163224 @default.
• W2163041776 cites W1563205636 @default.
• W2163041776 cites W168937217 @default.
• W2163041776 cites W1964690753 @default.
• W2163041776 cites W1964881589 @default.
• W2163041776 cites W1974589277 @default.
• W2163041776 cites W1978563721 @default.
• W2163041776 cites W1982084168 @default.
• W2163041776 cites W1983200944 @default.
• W2163041776 cites W1984143105 @default.
• W2163041776 cites W1986598723 @default.
• W2163041776 cites W1987568997 @default.
• W2163041776 cites W1988064309 @default.
• W2163041776 cites W1988564243 @default.
• W2163041776 cites W1989446473 @default.
• W2163041776 cites W1991619717 @default.
• W2163041776 cites W1992053404 @default.
• W2163041776 cites W1996703250 @default.
• W2163041776 cites W2001027887 @default.
• W2163041776 cites W2006842464 @default.
• W2163041776 cites W2006848373 @default.
• W2163041776 cites W2009588677 @default.
• W2163041776 cites W2017812238 @default.
• W2163041776 cites W2022397399 @default.
• W2163041776 cites W2027581733 @default.
• W2163041776 cites W2030227542 @default.
• W2163041776 cites W2033458917 @default.
• W2163041776 cites W2035409937 @default.
• W2163041776 cites W2038087177 @default.
• W2163041776 cites W2041744828 @default.
• W2163041776 cites W2042282321 @default.
• W2163041776 cites W2044170628 @default.
• W2163041776 cites W2048989707 @default.
• W2163041776 cites W2054703329 @default.
• W2163041776 cites W2055059608 @default.
• W2163041776 cites W2059921402 @default.
• W2163041776 cites W2060572191 @default.
• W2163041776 cites W2062745430 @default.
• W2163041776 cites W2062953809 @default.
• W2163041776 cites W2063391415 @default.
• W2163041776 cites W2063753069 @default.
• W2163041776 cites W2067793264 @default.
• W2163041776 cites W2075913166 @default.
• W2163041776 cites W2078571162 @default.
• W2163041776 cites W2078631340 @default.
• W2163041776 cites W2078827619 @default.
• W2163041776 cites W2079104847 @default.
• W2163041776 cites W2081104479 @default.
• W2163041776 cites W2085867052 @default.
• W2163041776 cites W2085939108 @default.
• W2163041776 cites W2088404831 @default.
• W2163041776 cites W2089577337 @default.
• W2163041776 cites W2090417853 @default.
• W2163041776 cites W2091273852 @default.
• W2163041776 cites W2094598916 @default.
• W2163041776 cites W2096154667 @default.
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• W2163041776 cites W2118506472 @default.
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• W2163041776 cites W2160734895 @default.
• W2163041776 cites W2171868533 @default.
• W2163041776 cites W2188060873 @default.
• W2163041776 cites W2215241030 @default.
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• W2163041776 doi "https://doi.org/10.1677/joe.0.1690347" @default.
• W2163041776 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11312151" @default.
• W2163041776 hasPublicationYear "2001" @default.
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