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• W2169486392 abstract "ABSTRACT RND (resistance-nodulation-division) family transporters in Gram-negative bacteria frequently pump out a wide range of inhibitors and often contribute to multidrug resistance to antibiotics and biocides. An archetypal RND pump of Escherichia coli , AcrB, is known to exist as a homotrimer, and this construction is essential for drug pumping through the functionally rotating mechanism. MdtBC, however, appears different because two pump genes coexist within a single operon, and genetic deletion data suggest that both pumps must be expressed in order for the drug efflux to occur. We have expressed the corresponding genes, with one of them in a His-tagged form. Copurification of MdtB and MdtC under these conditions showed that they form a complex, with an average stoichiometry of 2:1. Unequivocal evidence that only the trimer containing two B protomers and one C protomer is active was obtained by expressing all possible combinations of B and C in covalently linked forms. Finally, conversion into alanine of the residues, known to form a proton translocation pathway in AcrB, inactivated transport only when made in MdtB, not when made in MdtC, a result suggesting that MdtC plays a different role not directly involved in drug binding and extrusion." @default.
• W2169486392 created "2016-06-24" @default.
• W2169486392 creator A5060279819 @default.
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• W2169486392 date "2010-03-01" @default.
• W2169486392 modified "2023-10-06" @default.
• W2169486392 title "Multidrug Efflux Pump MdtBC of <i>Escherichia coli</i> Is Active Only as a B ₂ C Heterotrimer" @default.
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• W2169486392 doi "https://doi.org/10.1128/jb.01448-09" @default.
• W2169486392 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2820868" @default.
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