FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2210369950> ?p ?o ?g. }

• W2210369950 endingPage "47" @default.
• W2210369950 startingPage "42" @default.
• W2210369950 abstract "As the extracellular nuclease of Aspergillus, S1 nuclease can split single and double-stranded DNA into oligo- or mononucleotides, while preferentially digests single-stranded nucleic acids. Furthermore, the existence of S1 can be the standard to identify Aspergillus and used to evaluate the severity of Aspergillosis. Herein, a simple and sensitive fluorescent sensing platform for S1 assay was developed based on the S1-induced DNA strand scission and the difference in affinity of graphene oxide (GO) for single-stranded DNA containing different bases. This platform was applied to monitor S1 activity and study the kinetics in real time. Results indicated that the detection limit is 0.5 U/mL. The Km and kcat at 45 °C, are 1.4 ± 0.12 μM and 0.6 min− 1, respectively. Moreover, by monitoring the effect of chemical drugs on S1 activity, we found that 2 mM of erythromycin, sodium penicillin, carbenicillin disodium and ampicillin can inhibit S1 activity about 8%, 60%, 61% and 66%, respectively, while gentamycin sulfate is a stimulator. Overall, the assay platform based on graphene oxide quenched fluorescence probe is successfully constructed to study the enzymatic activity of S1 and used for screening antibiotics." @default.
• W2210369950 created "2016-06-24" @default.
• W2210369950 creator A5007466711 @default.
• W2210369950 creator A5032243659 @default.
• W2210369950 creator A5050000000 @default.
• W2210369950 creator A5064401891 @default.
• W2210369950 creator A5067548058 @default.
• W2210369950 creator A5090079152 @default.
• W2210369950 date "2016-03-01" @default.
• W2210369950 modified "2023-10-03" @default.
• W2210369950 title "A real time S1 assay at neutral pH based on graphene oxide quenched fluorescence probe" @default.
• W2210369950 cites W1538861409 @default.
• W2210369950 cites W1964022191 @default.
• W2210369950 cites W1966736575 @default.
• W2210369950 cites W1972770657 @default.
• W2210369950 cites W1974954646 @default.
• W2210369950 cites W1975837635 @default.
• W2210369950 cites W1981563140 @default.
• W2210369950 cites W1987288969 @default.
• W2210369950 cites W1989057809 @default.
• W2210369950 cites W2011115824 @default.
• W2210369950 cites W2012496589 @default.
• W2210369950 cites W2025716631 @default.
• W2210369950 cites W2033400692 @default.
• W2210369950 cites W2041029698 @default.
• W2210369950 cites W2045466817 @default.
• W2210369950 cites W2051782407 @default.
• W2210369950 cites W2080645389 @default.
• W2210369950 cites W2081946703 @default.
• W2210369950 cites W2082572892 @default.
• W2210369950 cites W2139517150 @default.
• W2210369950 cites W2155795394 @default.
• W2210369950 cites W2171409050 @default.
• W2210369950 cites W4229859845 @default.
• W2210369950 doi "https://doi.org/10.1016/j.sbsr.2015.12.002" @default.
• W2210369950 hasPublicationYear "2016" @default.
• W2210369950 type Work @default.
• W2210369950 sameAs 2210369950 @default.
• W2210369950 citedByCount "5" @default.
• W2210369950 countsByYear W22103699502017 @default.
• W2210369950 countsByYear W22103699502020 @default.
• W2210369950 countsByYear W22103699502023 @default.
• W2210369950 crossrefType "journal-article" @default.
• W2210369950 hasAuthorship W2210369950A5007466711 @default.
• W2210369950 hasAuthorship W2210369950A5032243659 @default.
• W2210369950 hasAuthorship W2210369950A5050000000 @default.
• W2210369950 hasAuthorship W2210369950A5064401891 @default.
• W2210369950 hasAuthorship W2210369950A5067548058 @default.
• W2210369950 hasAuthorship W2210369950A5090079152 @default.
• W2210369950 hasBestOaLocation W22103699501 @default.
• W2210369950 hasConcept C119128265 @default.
• W2210369950 hasConcept C121332964 @default.
• W2210369950 hasConcept C13965031 @default.
• W2210369950 hasConcept C171250308 @default.
• W2210369950 hasConcept C178790620 @default.
• W2210369950 hasConcept C181199279 @default.
• W2210369950 hasConcept C185592680 @default.
• W2210369950 hasConcept C192562407 @default.
• W2210369950 hasConcept C21951064 @default.
• W2210369950 hasConcept C2776021129 @default.
• W2210369950 hasConcept C2777271071 @default.
• W2210369950 hasConcept C2778484023 @default.
• W2210369950 hasConcept C2779787849 @default.
• W2210369950 hasConcept C2779851234 @default.
• W2210369950 hasConcept C2779913634 @default.
• W2210369950 hasConcept C30080830 @default.
• W2210369950 hasConcept C41183919 @default.
• W2210369950 hasConcept C43617362 @default.
• W2210369950 hasConcept C501593827 @default.
• W2210369950 hasConcept C552990157 @default.
• W2210369950 hasConcept C55493867 @default.
• W2210369950 hasConcept C56856141 @default.
• W2210369950 hasConcept C62520636 @default.
• W2210369950 hasConcept C86803240 @default.
• W2210369950 hasConcept C89423630 @default.
• W2210369950 hasConcept C91881484 @default.
• W2210369950 hasConceptScore W2210369950C119128265 @default.
• W2210369950 hasConceptScore W2210369950C121332964 @default.
• W2210369950 hasConceptScore W2210369950C13965031 @default.
• W2210369950 hasConceptScore W2210369950C171250308 @default.
• W2210369950 hasConceptScore W2210369950C178790620 @default.
• W2210369950 hasConceptScore W2210369950C181199279 @default.
• W2210369950 hasConceptScore W2210369950C185592680 @default.
• W2210369950 hasConceptScore W2210369950C192562407 @default.
• W2210369950 hasConceptScore W2210369950C21951064 @default.
• W2210369950 hasConceptScore W2210369950C2776021129 @default.
• W2210369950 hasConceptScore W2210369950C2777271071 @default.
• W2210369950 hasConceptScore W2210369950C2778484023 @default.
• W2210369950 hasConceptScore W2210369950C2779787849 @default.
• W2210369950 hasConceptScore W2210369950C2779851234 @default.
• W2210369950 hasConceptScore W2210369950C2779913634 @default.
• W2210369950 hasConceptScore W2210369950C30080830 @default.
• W2210369950 hasConceptScore W2210369950C41183919 @default.
• W2210369950 hasConceptScore W2210369950C43617362 @default.
• W2210369950 hasConceptScore W2210369950C501593827 @default.
• W2210369950 hasConceptScore W2210369950C552990157 @default.
• W2210369950 hasConceptScore W2210369950C55493867 @default.
• W2210369950 hasConceptScore W2210369950C56856141 @default.

• next