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- W2520939061 abstract "We studied whether the accumulation of advanced lipoxidation end-products (ALEs) in the diabetic retina is linked to the impairment of lipid aldehyde detoxification mechanisms.Retinas were collected from nondiabetic and diabetic rats and processed for conventional and quantitative RT-PCR (qRT-PCR), Western blotting, immunohistochemistry, and aldehyde dehydrogenase (ALDH) activity assays. The effect of the ALDH1a1 inhibitor, NCT-501, on ALE accumulation and cell viability in cultured Müller glia also was investigated.The rat retina expressed a range of lipid aldehyde detoxifying ALDH and aldo-keto reductase (AKR) genes. In diabetes, mRNA levels were reduced for 5 of 9 transcripts tested. These findings contrasted with those in the lens and cornea where many of these enzymes were upregulated. We have reported previously accumulation of the acrolein (ACR)-derived ALE, FDP-lysine, in retinal Müller glia during diabetes. In the present study, we show that the main ACR-detoxifying ALDH and AKR genes expressed in the retina, namely, ALDH1a1, ALDH2, and AKR1b1, are principally localized to Müller glia. Diabetes-induced FDP-lysine accumulation in Müller glia was associated with a reduction in ALDH1a1 mRNA and protein expression in whole retina and a decrease in ALDH1a1-immunoreactivity specifically within these cells. No such changes were detected for ALDH2 or AKR1b1. Activity of ALDH was suppressed in the diabetic retina and blockade of ALDH1a1 in cultured Müller glia triggered FDP-lysine accumulation and reduced cell viability.These findings suggest that downregulation of ALDH and AKR enzymes, particularly ALDH1a1, may contribute ALE accumulation in the diabetic retina." @default.
- W2520939061 created "2016-09-23" @default.
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- W2520939061 date "2016-09-12" @default.
- W2520939061 modified "2023-09-30" @default.
- W2520939061 title "Diabetes Impairs the Aldehyde Detoxifying Capacity of the Retina" @default.
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- W2520939061 cites W1207588145 @default.
- W2520939061 cites W1497442006 @default.
- W2520939061 cites W1544906050 @default.
- W2520939061 cites W182664335 @default.
- W2520939061 cites W1841830236 @default.
- W2520939061 cites W1968332094 @default.
- W2520939061 cites W1968484754 @default.
- W2520939061 cites W1969768095 @default.
- W2520939061 cites W1976546025 @default.
- W2520939061 cites W1978495960 @default.
- W2520939061 cites W1978625417 @default.
- W2520939061 cites W1981144202 @default.
- W2520939061 cites W1983704379 @default.
- W2520939061 cites W1995007556 @default.
- W2520939061 cites W1995322279 @default.
- W2520939061 cites W2007017828 @default.
- W2520939061 cites W2007686989 @default.
- W2520939061 cites W2009843396 @default.
- W2520939061 cites W2012928161 @default.
- W2520939061 cites W2013459762 @default.
- W2520939061 cites W2021584836 @default.
- W2520939061 cites W2023534921 @default.
- W2520939061 cites W2032849378 @default.
- W2520939061 cites W2039527984 @default.
- W2520939061 cites W2042672024 @default.
- W2520939061 cites W2043243208 @default.
- W2520939061 cites W2043250256 @default.
- W2520939061 cites W2045333865 @default.
- W2520939061 cites W2046275490 @default.
- W2520939061 cites W2048975500 @default.
- W2520939061 cites W2050138059 @default.
- W2520939061 cites W2054890136 @default.
- W2520939061 cites W2055707729 @default.
- W2520939061 cites W2061813962 @default.
- W2520939061 cites W2063141055 @default.
- W2520939061 cites W2064407997 @default.
- W2520939061 cites W2070001955 @default.
- W2520939061 cites W2071162782 @default.
- W2520939061 cites W2073502302 @default.
- W2520939061 cites W2078086567 @default.
- W2520939061 cites W2082495848 @default.
- W2520939061 cites W2085616178 @default.
- W2520939061 cites W2086137541 @default.
- W2520939061 cites W2086805984 @default.
- W2520939061 cites W2088950533 @default.
- W2520939061 cites W2096597598 @default.
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- W2520939061 cites W2107277218 @default.
- W2520939061 cites W2113845574 @default.
- W2520939061 cites W2121919167 @default.
- W2520939061 cites W2123686364 @default.
- W2520939061 cites W2124466520 @default.
- W2520939061 cites W2127126726 @default.
- W2520939061 cites W2132949972 @default.
- W2520939061 cites W2137102572 @default.
- W2520939061 cites W2138393164 @default.
- W2520939061 cites W2141234587 @default.
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- W2520939061 cites W2183747868 @default.
- W2520939061 cites W2193621263 @default.
- W2520939061 cites W2211950094 @default.
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- W2520939061 cites W2461889575 @default.
- W2520939061 cites W2949582036 @default.
- W2520939061 cites W1493355601 @default.
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- W2520939061 doi "https://doi.org/10.1167/iovs.16-19424" @default.
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- W2520939061 hasPublicationYear "2016" @default.
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