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• W2580459214 abstract "The development of transgenic technologies in the Cashmere goat (Capra hircus) has the potential to improve the quality of the meat and wool. The piggyBac (PB) transposon system is highly efficient and can be used to transpose specific target genes into the genome. Here, we developed a PB transposon system to produce transgenic Cashmere goat fetal fibroblasts (GFFs) with the enhanced green fluorescent protein (EGFP). We then used the genetically modified GFFs as nuclear donors to generate transgenic embryos by somatic cell nuclear transfer (SCNT). The embryos (n = 40) were implanted into female goats (n = 20). One transgenic kid that expressed EGFP throughout the surface features of its body was born. This result demonstrated the usefulness of PB transposon system in generating transgenic Cashmere goats." @default.
• W2580459214 created "2017-02-03" @default.
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• W2580459214 date "2017-04-01" @default.
• W2580459214 modified "2023-09-23" @default.
• W2580459214 title "Generation of a transgenic cashmere goat using the piggyBac transposition system" @default.
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• W2580459214 doi "https://doi.org/10.1016/j.theriogenology.2017.01.036" @default.
• W2580459214 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28257857" @default.
• W2580459214 hasPublicationYear "2017" @default.
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