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• W2626374941 abstract "Streptococcus agalactiae is the leading cause of early-onset neonatal sepsis. Culture-based screening methods lack the sensitivity of molecular assays and do not indicate serotype; a potentially important virulence marker. We aimed to develop a multiplex PCR to detect S. agalactiae while simultaneously identifying serotypes Ia, Ib, and III; commonly associated with infant disease. Primers were designed to target S. agalactiae serotype-specific cps genes and the dltS gene. The assay was validated with 512 vaginal specimens from pregnant women. 112 (21.9%) were dltS positive, with 14.3%, 0.9%, and 6.3% of these identified as cps Ia, Ib, and III, respectively. Our assay is a specific and sensitive method to simultaneously detect S. agalactiae and serotypes Ia, Ib, and III in a single step. It is of high significance for clinical diagnostic applications and also provides epidemiological data on serotype, information that may be important for vaccine development and other targeted non-antibiotic therapies." @default.
• W2626374941 created "2017-06-23" @default.
• W2626374941 creator A5003029351 @default.
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• W2626374941 date "2017-09-01" @default.
• W2626374941 modified "2023-09-23" @default.
• W2626374941 title "A novel one-step real-time multiplex PCR assay to detect Streptococcus agalactiae presence and serotypes Ia, Ib, and III" @default.
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• W2626374941 doi "https://doi.org/10.1016/j.diagmicrobio.2017.06.003" @default.
• W2626374941 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28669679" @default.
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